Dinesh Kumar1*, R.P.S. Dalal1, Rakesh Kumar2, Jagdish Prashad2 and Jaipal jaipal1
Department of Horticulture1, Department of Microbiology2, CCS Haryana Agricultural University, Hisar, Haryana, India-125004
email: palri.ado@gmail.com
Received-07.07.2021, Revised-20.07.2021, Accepted-29.07.2021
Abstract: The aim of the present study was to analyze seasonal variations in phyllospheric microbial biomass of different fruit tree species. Leaf samples were collected from seven fruit tree orchards viz. mango, guava, aonla, ber, bael, jamun, sweet orange during summer, rainy and winter season for estimation of microbial population. In phyllosphere of different fruit tree orchards maximum TBC was observed in mango during summer, jamun during rainy, aonla and sweet orange during winter season. Highest diazotrophs were observed in aonla during summer and in jamunphyllosphere during rainy and winter season. Microbial populations and fungal count decreased by 13.06 % to 42.02 % from summer to rainy season, whereas increased by 10.80 % to 32.39 % from rainy to winter season in respect to phyllosphere of all the fruit tree species.
Keywords: Fruit tree species, Phyllosphere, Microbial Population
RefErences
Afifi, M.M.I., El-Sayed, G.A.M., Manal, A.H. El-Gamal and Massoud, O.N. (2014).Synergistic Effect of Biofertilizers containing N-fixer, P and K Solubilizers and Humic Substances on Sorghum bicolor Productivity. Middle East J. of App.Sci.4(4):1065-1074.
Andrews,J.H. and Harris,R.F.(2000).Theecologyandbiogeographyof microorganisms on plant surfaces.Annu. Rev.Phytopathol.38:145–180.
Batool, F., Rehman, Y. and Hasnain, S.(2016). phylloplane associated plant bacteria of commercially superior wheat varieties exhibit superior plant growth promoting abilities. Fro.in life sci. 9(4): 313-322.
Beattie, G.A. and Lindow, S.E.(1995).Thesecretlifeoffoliarbacterialpathogensonleaves.Annu Rev Phytopathol.33: 145–172.
Ercolani, GL.(1991). Distribution of epiphytic bacteria on olive leaves and the influence of leaf age and sampling time.Microbial Eco.21: 35–48.
Hunter, P.J., Hand, P., Pink, D., Whipps, J.M. and Bending, G.D.(2010). Both leaf properties and microbe-microbe interactions influence within-species variation in bacterial population diversity and structure in the Lettuce (Lactuca species) phyllosphere. Appl. Environ. Microbiol.76: 8117–8125.
Jackson, E.F.Echlin, H.L. and Jackson, C.R.(2006).Changes in the phyllosphere community of the resurrection fern, Polypodiumpolypodioides, associated with rainfall and wetting.FEMS Microbiol.Eco.58: 236–246.
Jacques, M.A. and Morris,C.E.(1995).A review of issues related to the quantification of bacteria from the phyllosphere. FEMS Microbiol.Eco.18: 1–14.
Jager, F.C. Wehner, F.C. and Korsten, L.(2001).Microbial Ecology of the Mango Phylloplane.Micro.Eco.42: 201–207.
Kadivar, H. and Stapleton, A.E.(2006). Ultraviolet radiation alters maize phyllosphere bacterial diversity. Microbial Ecology.45: 353–361.
Lindow, S. and Andersen, G.(1996).Influence of Immigration on Epiphytic Bacterial Populations on Navel Orange Leaves.Applied and Environmental Microbiology.62(8): 2978–298.
Lindow, S. E. and Brandl, M. T. (2003).Microbiology of the phyllosphere.Appl. Environ. Microbiol.69:1875-1883.
Miyamoto, T.,Kawahara, M. and Minamisawa, K.(2004).Novelendophytic nitrogen-fixing clostridia from the grass Miscanthussinensis as revealed by terminal restriction fragment length polymorphism analysis. Appl Environ Microbiol.70: 6580–6586.
National Horticulture Board.Indian horticulture database(2017-18). National Horticulture Board, Ministry of Agriculture, Government of India. Retrieved from http://nhb.gov.in/pdf/Annual%20Report%202017-18.pdf
Oliveira, A.N. and Luiz Antonio de Oliveira, L.A.(2005).Seasonal dynamics of arbuscularmycorrhizal fungi in plants of theobromagrandiflorumschum and paulliniacupanamart. Of an agroforestry system in central amazonia, amazonas state, Brazil.Brazilian Journal of Microbiology. 36:262-270.
Pennycook, S.R. and Newhook, F.J.(1981).Seasonal changes in the apple phylloplanemicroflora. New Zealand Journal of Botany.19:273-283.
Pense, V.G. and Sukhatme, P.V.(1987).Statistical methods for agricultural workers.IInd Ed. I.C.A.R. Agric. Circ. No. 843.
Rasche, F. Trondl, R. Naglreiter, C. Reichenauer, T. and Sessitsch, A.(2006).Chilling and cultivar type affect the diversity of bacterial endophytes colonizing sweet pepper (Capsicum anuum L.). Can. J. Microbiol.52: 1036–1045.
Rastogi, G., Sbodio, A., Tech, J.J., Suslow, T.V., Coaker, G.L. and Leveau, J.H.(2012).Leaf microbiota in an agroecosystem: spatio temporal variation in bacterial community composition on field-grown lettuce.,ISME J, 6:1812–1822.
Stapleton, A.E. and Simmons, S.J.(2006).Plant control of phyllosphere diversity: genotype interactions with ultraviolet B radiation, In Microbial Ecology of the Aerial Plant Surface. Wallingford, UK: CABI International.pp. 223–238.
Whipps, J.M., Hand, P., Pink, D. and Bending, G.D.(2008). Phyllosphere microbiology with special reference to diversity and plant genotype.J.App.Microbiol.105: 1744 – 1755.