Meena Choudhary*, Sarita Aryaand Inder Dev Arya
Genetics and Tree Improvement Division,
Arid Forest Research Institute, Jodhpur, Rajasthan, India
Received-24.09.2021, Revised-16.10.2021, Accepted-24.10.2021
Abstract: Realizing the immense potential of Terminalia arjuna as a source of valuable medicine and considering its ever-increasing demand the present study was taken up to highlight the most crucial step of any micropropagation protocol i. e. in vitro shoot multiplication as it determines the availability of shoot stock for rooting or complete plantlet formation. With increase in shoot multiplication fold, success of micropropagation also increases. Thus, to maximize in vitro shoot multiplication many factors, in addition to plant growth hormone, are also important. Present study concluded that propagule size for subculture, type of growth medium, medium strength, carbohydrate source and subculture duration also affect shoot multiplication. Propagule with three shoots were cultured on modified MS medium (MMS) fortified with 4.44 μM BAP + 0.54 μM NAA + additives and 3% sucrose gave 3.7 fold shoot multiplication after 4 weeks.
Keywords: Terminalia arjuna, in vitro shoot multiplication, modified MS medium, propagule, carbohydrate
REFERENCES
Arora, K., Sharma, M., Srivastava, J., Ranade, S.A. and Sharma, A.K. (2010). Rapid in vitro cloning of a 40-year-old tree of Azadirachta indica A. Juss. (Neem) employing nodal stem segments. Agroforestry Systems, 78(1): 53-63.
Arya, I.D. and Arya, S. (1997). In vitro culture and establishment of exotic bamboo Dendrocalamus asper. Indian Journal of Experimental Biology, 35: 1252-1255.
Arya, I.D., Kaur, B. and Arya, S. (2012). Rapid and mass propagation of economically important Bamboo Dendrocalamus hamiltonii. Indian Journal of Energy, 1 (1): 11-16.
Arya, S., Rana, P.K., Sharma, R. and Arya, I.D. (2006). Tissue culture technology for rapid multiplication of Dendrocalamus giganteus Munro. Indian Forester, 3: 345-357.
Bonga, J.M. and Aderkas, V.P. (1992). Clonal propagation. In: Bonga, J.M. and Aderkas, V.P. (Eds.). In vitro culture of trees, Kluwer Academic Publishers, Boston, pp. 72-125.
Choudhary, M., Arya, I.D. and Arya, S. (2020). Factors affecting ex vitro rooting in micro propagated shoots from nodal explants of Terminalia arjuna. Current Journal of Applied Science and Technology, 39:92-97.
Choudhary, M., Gehlot, A., Arya, I.D. and Arya, S. (2018). Influence of different auxin treatment on ex vitro rooting in in vitro regenerated micro shoots of Terminalia arjuna (Arjun). Journal of Pharmacognosy and Phytochemistry, 7:3079-3082.
Choudhary, M., Jaiswal, S, Singh, R, Arya, I.D. and Arya, S.A. (2015). Micropropagation protocol for mass multiplication of Terminalia arjuna – a valuable medicinal tree. Advances in Forestry Science, 2:1-6.
Gamborg, O.L., Miller, R.A. and Ojima, K. (1968). Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research, 50: 151–158.
Goyal, Y. and Arya, H.C. (1984). Effect of sugars, Nitrogen, amino acids and vitamins on shoot differentiation, from single bud in vitro bud culture of Prosopis cineraria L. Indian Journal of Experimental Biology, 22: 592-595.
Gupta, A.K., Harish, Rai, M.K., Phulwaria, M., Agarwal, T. and Shekhawat, N.S. (2014). In vitro propagation, encapsulation, and genetic fidelity analysis of Terminalia arjuna: a cardioprotective medicinal tree. Applied Biochemistry and Biotechnology, 173 (6): 1481-1494.
Husain, M.K. and Anis, M. (2009). Rapid in vitro multiplication of Melia azedarach L. (a multipurpose woody tree). Acta Physiologiae Plantarum, 31: 765-772.
Lloyd, G. and McCown, B.H. (1981). Commercially-feasible micropropagation of Mountain Laurel, Kalmia latifolia, by shoot tip culture. Combined Proceedings International Plant Propagations Society, 30: 421-427.
Maulik, S.K., Wilson, V., Seth, S., Bhargava, B., Dua, P., Ramakrishnan, S. and Katiyar, C.K. (2016). Clinical efficacy of water extract of stem bark of Terminalia arjuna (Roxb. ex DC.) Wight & Arn. in patients of chronic heart failure: a double-blind, randomized controlled trial. Phytomedicine, 23: 1211–1219.
Meghwani, H., Prabhakar, P., Mohammed, S.A., Seth, S., Hote, M.P., Banerjee, S.K., Arava, S., Roy, R. and Maulik, S.K. (2017). Beneficial effects of aqueous extract of stem bark of Terminalia arjuna (Roxb.), an ayurvedic drug in experimental pulmonary hypertension. Journal of Ethnopharmacology, 197: 184–194.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.
Pandey, S. and Jaiswal, V.S. (2002). Micropropagation of Terminalia arjuna Roxb. from cotyledonary nodes. Indian Journal of Experimental Biology, 40: 950- 953.
Pandey, S., Singh, M., Jaiswal, U. and Jaiswal, V.S. (2006). Shoot initiation and multiplication from a mature tree of Terminalia arjuna roxb. In Vitro Cellular and Developmental Biology- Plant, 42: 389-393.
Phulwaria, M., Rai, M.K., Harish, Gupta, A.K., Ram, K. and Shekhawat, N.S. (2012). An improved micropropagation of Terminalia bellirica from nodal explants of mature tree. Acta Physiologiae Plantarum, 34: 299-305.
Prutpongse, P. and Gavinlertvatana, P. (1992). In vitro micropropagation of 54 species from 15 genera of bamboo. Hort Science, 27:453-454.
Siddique, I. and Anis, M. (2007). In vitro shoot multiplication and plantlet regeneration from nodal explants of Cassia angustifolia (Vahl.): a medicinal plant. Acta Physiologiae Plantarum, 29(3): 233-238.
Singh, A.P.P. and Chauhan, S.M.S. (2014). Activity-guided isolation of antioxidants from the leaves of Terminalia arjuna. Natural Product Research, 28(10): 760-763.
Tuskan, G.A., Sargent, W.A., Rensema, T. and Walla, J.A. (1990). Influence of plant growth regulators, basal media, and carbohydrate level on the in vitro development of Pinus ponderosa (Dougl. ex Law.) cotyledon explants. Plant Cell, Tissue and Organ Culture, 20: 47-52.
Verma, A. and Singh, A. (2013). Optimization of ingredients for a herbal beverage with medicinal attributes. Asian Journal of Dairy and Food Research, 32(4): 318-322.