Sharda Choudhary*, R.S. Meena, Geetika Jethra, Radheshyam Sharma and Alka Panwar
National Research Centre on Seed Spices, Tabiji, Ajmer, India – 305 206
Received-27.12.2014, Revised-03.01.2015
Abstract: In this study, an efficient, simple and rapid protocol is described for high quality DNA isolation from leaves and seeds of fennel (Foeniculum vulgare). The protocol gives highly reproducible results and can be carried out easily. Young leaves and seeds of fennel were kept at -80º C for 20 min to freeze the tissues and make the grinding easy without any tissue damage. This protocol eliminates the use of liquid nitrogen. The protocol is inspired by the CTAB method and Sambrook principles.
Keywords: Seed spices, DNA, Fennel, Seeds, Leaves
REFERENCES
Bruyas-Bertholon, V.; Lachaux, A.; Dubois, J.P.; Fourneret, P. and Letrilliart, L. (2012). Essential oil composition and antifungal activity of Foeniculum vulgare Mill. Obtained by different distillation conditions. Agricultural and Food Chemistry. 54(18): 6814-6820.
Krizman, M.; Jernej J.; Dea B.; Branka, J. and Mirko, P. (2006). Robust CTAB activated charcoal protocol for plant DNA extraction. Acta Agriculturae Slovencia: 87 (2): 427-433.
Leninger, A. L. (1975). Biochemistry, 2nd ed., Worth Publishers, New York.
Lucinewton, S.; Raul, N.; Carvalho, J.; Mirian, B.; Lin, C. and Angela, A. (2005). Supercritical fluid extraction from fennel, (Foeniculum vulgare), global yield, composition and kinetic data. Journal of Supercritical Fluid. 35: 212-219.
Oktay M.; Gulcin, I. and Kufrevioglu, O.I. (2003). Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts. LWT-Food Science and Technology. 36(2): 263-271.
Porebski, S.; Bailey, L.G. and Baum, B.R. (1997). Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Molecular Biology Reporter, 15:8-15.
Sambrook, J.; Fritsch, E.F. and Maniatis, T. (1989). Molecular cloning; a laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NewYork.
Saravanaperumal, S.A. and Terza A.L. (2012). Polyphenolics free DNA isolation and optimization of PCR-RAPD for fennel (Foeniculum vulgare Mill.) from mature and young leaves. African Journal of Biotechnology 11(35):8622-8631.