Vikas Sharma1*,Navneet Kour2, Arti Heer3, Sweeta Manhas4, Sheikh Manzoor Shafi5, Shashank K. Singh6 andShivangi Sharma7
1,2,3,4,5Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Faculty of Basic Sciences, Main Campus Chatha, Jammu-180 009, J&K, India
6Cancer Pharmacology Division, Indian Institute of Integrative Medicine,
Canal Road Jammu-180001, J&K, India
7Department of Chemistry, University of Jammu, Baba Saheb Ambedkar Road,
Jammu-180006, J&K, India
Email: vikas.skuast@gmail.com
Received-07.07.2018, Revised-26.07.2018
Abstract: The present research work was carried out to examine the in vitro cytotoxic effect of four minor fruits of kandi belt of Jammu region viz., Emblica officinalis (amla), Morinda citrifolia (shehtoot), Olea europaea (jaitun), Ziziphus mauritiana (ber) against nine human cancer cell lines from eight different origins such as A-549 (lung), A-498 (renal), HCT-116 (colon), MCF-7 (breast), MDA-MB-435 (melanoma), OVCAR-5 (ovarian), PC-3 (prostate), SF-295 (CNS), T-47D (breast). Methanolic extracts of fruits were used as test material and in vitro cytotoxicity was determined at 100 μg/ml via SRB assay. Results revealed that all the minor fruits (except shehtoot) showed in vitro cytotoxic efficacy against one or the other human cancer cell line with growth inhibition range of 70-99%. Maximum growth inhibition against more number of human cancer cell lines was produced by amla followed by ber and jaitun. When evaluated at lower concentrations, ber fruit exhibits significant in vitro cytotoxic potential (79% & 70%) at 50 and 30 μg/ml respectively against lung cancer cell line (A-549). Ber also suppressed 70% proliferation of melanoma cancer cell line (MDA-MB-435) at 50 μg/ml. To conclude, ber possesses certain constituents with cytotoxic properties that will be effective against melanoma and lung cancer cells.
Keywords: Amla, Ber, Cancer Cells, In vitro cytotoxicity, SRB assay
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