2021, Issue 10, Volume 13

IN-VITRO STUDIES ON COMPATIBILITY BEHAVIOR AMONG MICROBIAL INOCULANTS

Patil D.A.,* Patil G.A.,1 Joshi M.U.2 and Govekar Y.R.3

Department of Plant Pathology and Agricultural Microbiology, PGI, MPKV, Rahuri. 413-722

1Department of Botany, GGVP College, Halkarni, Kolhapur, Maharashtra

2 Department of Pl. Pathology and Agril. Microbiology, PGI, MPKV, Rahuri

3Department of Pl. Pathology, Dr. BSKKV, Dapoli

Email: deeppatil2211@gmail.com

Received-05.10.2021, Revised-20.10.2021, Accepted-26.10.2021

Abstract: The present study was conducted during 2017-19 at Department of Plant Pathology and Agricultural Microbiology, Post Graduate Institute, M.P.K.V., Rahuri-413722. All the microbes have to prove effective in colonization of the plant roots for efficient function under natural soil conditions. Compatibility between the PGPR microbes to colonize the root system without inhibiting each other is a pre-requisite for success of using multiple microbes in a crop field. In our study, among the different microbes tested for their compatibility in culture growth, the all species of bioinoculants were found to compatible the growth of other species as evident from the no zone of inhibition observed in the plates. All other microbes viz; Trichoderma viride, Pseudomonas fluorescens, Azotobacter chroococcum and Bacillus polymyxa on NA media were compatible with each other. These results have made us to choose the best two candidate bacteria for further studies.

Keyword: Bioinoculants, Compatible, PGPR microbes

REFERENCES

Anandaraj, B. and Leema Rose Delapierre (2010). A studies on influence of bio inoculants (Pseudomonas fluorescens, Rhizobium sp., Bacillus megaterium) in green gram. J. Bio. Sci. Tech., 1(2): 95-99.

Bagwan, N. B. (2010). Evaluation of Trichoderma compatibility with fungicides, pesticides, organic cakes and botanicals for integerated management of soil borne diseases of soybean [Glycine max (L.) Merril]. International Journal of Plant Protection, 3(2): 206-209.

Garikapati, Sateesh and Sivasakthivelan, P. (2013). Studies on the influence of bioinoculant consortium on chillies and its effects on soil health management, Int.J.Chem.Tech. Res. 5, pp3.

Higa, T. (1994). Effective microorganisms: A new dimension for nature Farming. p. 20-22. In: Parr JF, Hornick SB and Simpson ME (Eds), Proceedings of the Second International Conference on Kyusei Nature Farming. US Department of Agriculture, Washington DC, USA.

Kumar, Rakesh, Kumar, Rameshand Kumar, Prabha (2010). Effect of integrated use of chemical fertilizers, biofertilizers and biostimulants in gladiolus (Gladiolus grandiflorus L.) cv. Sancerre Progressive horticulture, Indian journals, 43(1): 149-152.

Latha, P., Anand, T., Prakasam, V., Jonathan, E. I., Paramathma, M. and Samiyappan, R. (2011). Combining PseudomonasBacillus and Trichoderma strains with organic amendments and micronutrient to enhance suppression of collar and root rot disease in physic nut. Applied Soil Ecology,49, pp 215-223

Madewar Ganesh Prabhakar (2013). Compatibility studies on Phosphate Solubilizing Microorganisms with Trichoderma spp.M.Sc.Thesis, MPKV, Rahuri.

Manjula, K., Kishore, G.K., Girish, A.G. and Singh, S.D. (2004). Combined application of Pseudomonas fluorescens and Trichoderma viride has an improved biocontrol activity against stem rot in groundnut. Pl. Pathol. J., 20(1): 75-80.