Kuldeep Jangid, Omender Sangwan and Sagar*
Department of Genetics & Plant Breeding, CCS Haryana Agricultural University, Hisar-1250041,2,3,
Email: sagararora470@gmail.com
Received-02.03.2022, Revised-14.03.2022, Accepted-27.03.2022
Abstract: 30 advance lines of desi cotton were used for this study. Total 22 polymorphic markers amplified 62 bands with an average of 2.81 bands per marker. The band fragment size ranged from 120-380 bp. The polymorphism information content (PIC) ranged from 0.234 to 0.767 with an average of 0.518. BNL4108 marker gave maximum polymorphism with PIC value 0.767. HD526 and HD535 are most similar accessions with highest value of similarity matrix, 0.956. NAU2336 revealed least polymorphism with PIC value 0.234 and 2 different allele sizes. Significant diversity was found among all 30 genotypes even at molecular level and all the 30 genotypes were grouped into 4 clustered by polymorphism information of 22 SSR markers.
Keywords: Desi cotton, Diversity, Hisar, NTSYS-PC, SSR markers
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