Supratik Palchoudhury1, Bikram Saha1, Shibu Das1, Mohan K. Biswas2 and Kajal K. Biswas*
1Advanced Centre for Plant Virology, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India
2Department of Plant Protection, Palli Siksha Bhavana,Visva-Bharati University, West Bengal, India
Email: drkkbiswas@yahoo.co.in
Received-04.07.2019, Revised-26.07.2019
Abstract: In the present study, an improved and efficient plant regeneration protocol of Kagzilime (Citrus aurantifolia) using epicotyl segment of in vitro grown seedlings was developed. Kagzilime seed sterilized with Bavistin @ 0.1% for 30 min followed by Mercuric chloride @ 0.1% for 15 min was found to be optimum to reduce the contamination and efficient seed germination. About 0.75-1.0 cm long epicotyl segments of in vitro grown 21 days old seedlings were found suitable explants for efficient plant regeneration. The best regeneration efficiency of 84% with 5 shoots/explant was obtained at BAP @ 2.0 mg/l. The higher efficiency of root induction of 60.60% with 4.40 roots/shoot was observed at lower concentration of NAA@ 0.5 mg/l. Over 90% of plantlets were acclimatized and grown at pot mixture of soil, sand and vermiculite @ 1:2:1 in greenhouse. The efficient regeneration protocol developed in this study will be useful for mass propagation of root stock, biological indexing of virus diseases, production of disease free elite planting material, plant transformation and in vivo expression of desired viral gene.
Keywords: Age of explants, Epicotyl segment, Kagzilime, Multiple shoots, Regeneration
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