VARIABLE SALINITY TOLERANCE IN ANABAENA SP. BHUAR002 THROUGH REGULATION OF ION UPTAKE AND PRODUCTION OF OSMOPROTECTANT

Aparna Rai*

Department of Botany, Banaras Hindu University (BHU), Varanasi 221005, India

Email: aparna.rai82@gmail.com

Received-11.01.2018, Revised-25.01.2018

Abstract: Filamentous, heterocyst-forming, diazotrophiccyanobacteriumAnabaena sp. BHUAR002 was isolated from usar (saline) land near Banaras Hindu Universitycampus, and grown routinely on Allen Arnonmedium. The growth of cyanobacteriumwas measured at various concentrations (upto 1000 mM) of different salt combinations, NaCl, NaCl+Na₂CO₃ (1:1) and NaCl+Na₂SO₄ (1:1) and found thatthe cyanobacterium tolerated the salinity of 500 mMNaCl, 700 mMNaCl+Na₂CO₃ and 1000 mMNaCl+ Na₂SO₄, indicating that elevated carbonate and sulphate support the growth of cyanobacterium under salinity and increase the tolerance range. Natural abundance ¹³C-NMR spectra chemical shifts showed sucrose as the osmoticum synthesized in NaCl and NaCl+Na₂CO₃ (1:1). However, synthesis of sucrose was not found in case of NaCl+Na₂SO₄ (1:1). Intracellular Na⁺concentration increases under different salt concentrations as compared to control. K⁺concentration also increases with increase of different salt concentration as compared to control is also an indication of acclimatization against salt stress; this type of ionic ratio was found in all three salt stress conditions. Intracellular Cl^– concentration was found minimum in case of NaCl+Na₂SO₄ as compared to NaCl and NaCl+Na₂CO₃ incubated cells.

Keywords: Intracellular ion concentration, Osmotic, Salinity, Tolerance range

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