Mekala Mallikarjun* and Anil S. Kotasthane
Department of Plant Pathology, College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya (IGKV), Raipur – 492012, Chhattisgarh, India
Email:mallikarjunmekala1991@gmail.com
Received-15.08.2017, Revised-26.08.2017
Abstract: Bacterial blight (BB) of rice (Oryza sativa) caused by Xanthomonas oryzae pv. oryzae (Xoo) is currently one of the most important diseases limiting rice production and it has become widespread in India. This disease was first noticed by the farmers of Japan in 1884 (Tagami and Mizukami 1962). Enhancing genetic resistance has proven to be the most effective control method for controlling the disease. Four bacterial blight (BB) resistance genes, Xa4, Xa7, xa13 and Xa21, were introgressed into an elite rice cultivar. Marker assisted selection was done using linked molecular markers for genes Xa4, Xa7, xa13 and Xa21. The ability to quickly and reliably select desirable material and to eliminate individuals that contain deleterious alleles is critical to the success of the plant breeding program (Dubcovsky 2004). We report here in two gene pyramids Xa7+ Xa21 in 5 lines and Xa4+Xa7 in 1 line. Genes in combinations were found to provide high levels of resistance. Besides pyramids a set of 8 genotypes with only Xa7, 2 genotypes with only Xa13 and 5 genotypes for Xa21 were also found in this study. High resolution maps generated in-silico around Xa4, Xa7, xa13 and Xa21 can be useful for the precise placement of a gene of interest,analysis of regional and sub regional rates of recombination and appropriate combinations of markers for marker assisted selection.
Keywords: Gene Pyramiding, Bacterial blight, Resistance genes, Polymerase chain reaction, Marker Assisted Selection Xanthomonas oryzae pv. oryzae, MAS, Pyramiding
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