Prashant Singh1, 7, Deep Chandra Suyal2, Naveen Kumar3, Manish Dev Sharma4, Indra Rautela5, Aditya Patel,6 Narottam Sharma7 and Veena Pandey1
1Department of Biotechnology, Bhimtal Campus, Kumaun University, Nainital, Uttarakhand
2Department of Microbiology, Govind Ballabh Pant University of Agriculture &
Technology, Pantnagar, Uttarakhand.
3Molecular Cytogenetics Laboratory, Department of Molecular Biology & Genetic Engineering,Govind Ballabh Pant University of Agriculture & Technology Pantnagar.
4Department of Life Sciences, Shri Guru Ram Rai Institute of technology & Science, Dehradun
5Uttaranchal College of Applied and Life Sciences, Uttaranchal University, Dehradun
6Devsthali Vidhyapeeth, Lalpur, U S Nagar
7Central Molecular Research Laboratory, Biochemistry Department, Shri Guru Ram Rai Institute of Medical & Health Sciences, Dehradun
Email: Veena_kumaun@yahoo.co.in
Received-10.02.2017, Revised-02.03.2017
Abstract: The objective of this study was to isolate and characterize a rhizospheric bacterium from Munsyari, (2200 feet, 30.06oN/80.23o E) Uttarakhand, western Himalayas, (India). Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. Isolated strain was tested for various PGP traits like 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity, phosphate solubilisation, indole acetic acid production, production of siderophore, carbohydrate utilization test. Bio-control ability of isolate was also screened. Further identification of isolate was performed by PCR based 16S rRNA gene sequencing. The isolate PS03 was found to be most effective.
Keywords: Rhizobacteria, Isolation, Molecular, Himalaya region
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