2011, Issue-3 & 4, Volume 3

CYTOPATHOLOGICALLY STUDY OF JATROPHA CURCAS INFECTED WITH JATROPHA MOSAIC DISEASE

Sanjay Kumar, Rajeshwari Sharma, A.K. Sharma and Manoj Kr. Sharma

Department of Botany J.V. College, Baraut (Baghpat) U.P

Abstarct: Plant of Jatropha curcas infected with Jatropha mosaic disease by the whitefly (Bemisia tabaci), were studied cytopathologically. Plant leaves with typical symptoms exhibited the nucleopathic effects that are the characteristic of known whitefly-transmitted geminiviruses. Electron-dense fibrilar bodies occurred at an early stage of infection and were closely associated with virus like particales at a late stage of infection. Cytoplasmin inclusions which have membrane bound oval bodies containing granular or fibrilar material were also observed. Presence of fibrilar bodies suggested that Jatropha mosaic disease caused by a whitefly-transmitted geminivirus.

Keywords: Electronmicroscopy, cytopathology, Jatropha curcas

REFERENCES

Bird, J. (1957). A whitefly-transmitted mosaic of Jatropha gossipifilia, P.R. Agric. Exp. Stn.Tech. Pap. 22.

Christie, R. G., and Edwardson, J.R. (1977). Light and electron microscopy of plant virus Inclusion. Fla. Agric. Exp. Stn. Monogr. Ser.9.

Christie, R. G., Ko, N.J., Falk, B.W., Hiebert, E., Lastra, R., Bird. J. and Kim, K.S. (1986). Light microscopy of geminivirus-induced nuclear inclusion bodies. Phytopathology 76: 124-126.

Goodman, R.M. (1981). Geminiviruses. Pages 879-910 in: Hand book of plant virus Infections. E. Kurstak. Ed. Eisevier North-Holland Biomedical press New York.

Kim, K.S. and Flores, E.M. (1979). Nuclear changes associated with Euphorbia mosaic virus. Transmitted by whitefly. Phytopathology 69:980-984.

Kim, K.S., Shock, T.L. and Goodman. R.M. (1978). Infection of Phaseolus vulgaris by bean. Golden mosaic virus: Ultrastructural aspect. Virology 89:22-23.

Lastra, R. and Gil. F. (1981). Ultrastructural host cell changes associated with tomato yellow. Mosaic. Phytopathlogy 71:524-528.

Matthews, R.E.F. (1982). Classification and nomenclature of viruses. Intervirology 17:22-33.

Russo, M., Cohen, S. and Martelli, G.P. (1980). Virus-like particles in tomato plants infected. By the yellow leaf curl disease. J. Gen. Virol. 49:209-213.

Simons, J. N. and Coe, D. M. (1958). Transmission of pseudo-curly top virus in Florida by a Treehopper. Virology 6:43-48.

Thongmeearkom, P., Honda, Y., Saito, Y. and Syamanada, R. (1981). Nuclear Ultrastructural Changes and aggregates of virus-like particles in mungbean cells infected with mungbean yellow mosaic disease. Phytopathlogy, 71:41-44.