2014, Issue 3, Volume 6

MOLECULAR CHRACTERIZATION OF CHRYSANTHEMUM (CHRYSANTHEMUM MORIFOLIUM RAMAT) GERMPLASM USING RAPD  MARKERS

Deeksha Baliyan, Anil Sirohi, Devi Singh, Mukesh Kumar, Sunil Malik
and Manoj Kumar Singh

Sardar Vallabhbhai University of Agriculture & Technologym Meerut 250110 (UP), India

Abstract: Genetic variation among 24 chrysanthemum cultivars was examined by RAPD markers. A total of 79 fragments was produced with 10 RAPD primers and out of which 64 (81.01%) were found polymorphic and 15 bands (18.99%) monomorphic. The number of polymorphic fragments varied from 4.0 (OPF13) to 15 (OPF06) with an average of 7.9 bands per primer. The PIC was varied from 0.10 to 0.66 with an average .50, MI varied from o.36 to 6.99 with an average 2.92 and RP value was noted in the ranged from 5.17 to 14.50 with an average 9.40. UPGMA clustering revealed two major group ( Group1 and Group 2) and these further devided into seven clusters. Among the 24 genotypes, Poncho, Terri, Rangoli, Sweta, Ravikiran and Nanco are divergent and may be useful for breeding programme. Results suggested that RAPDs are highly useful for assessing the genetic diversity analysis among the chrysanthemum germplasm and parental selection studies in chrysanthemum.

Keywords: Chrysanthemum, molecular chracterization, RAPD markers, genetic diversity

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