Anamika Tripathi1, N.S. Abbas2 and Amrita Nigam1
1School of Sciences, Indira Gandhi National Open University, Maidan Garhi, New Delhi
2Department of Biology, Bhaskaracharya College of Applied Sciences, University of Delhi, New Delhi
Email: dr_nsabbas@yahoo.co.in
Abstract: An efficient protocol has been developed for rapid micropropagation of Ocimum citriodorum Vis., an endangered medicinal herb. The cotyledons were excised from the in vitro germinating seedlings and used as explants for the present study. The explants yielded the highest frequency of 87.49% shoot regeneration with an average shoot length of 4.98 cm on Murashige and Skoog (MS) medium supplemented with 1 mg l-1 6- benzylamino purine (BAP) + 0.1 mg l-1 napthalene acetic acid (NAA) + 500 mg l-1 casein hydrolysate (CH) + 25 mg l-1 adenine sulphate (AS). Alteration from the optimal concentration of BAP resulted in the formation of callus. Regenerated microshoots were separated and rooted on MS medium containing NAA (0.5 mg l-1). Well-developed complete plantlets were transferred onto plastic cups containing sterile soil and humus (1:1). Subsequently the acclimatized plantlets were successfully grown in garden. The regenerated plants were morphologically identical and exhibited similar growth characteristics as compared to the donor plants. Cytological studies of the regenerants revealed no change in chromosome numbers. Thus, regeneration protocol demonstrated in the present study provides a basis for the germplasm conservation and investigation of its medicinally active constituents.
Keywords: Cotyledonary explant, cytology, histological observations, ocimum citriodorum
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