Manoj Kumar Sharma, Astha Duhan* and Rajeshwari Sharma
*Department of Bioinformatics, J.V. College, Baraut, Baghpat (U.P.)
**Department of Bioinformatics, Mata Gujri College, Fatehgarh Sahib (Punjab).
Abstract: Expressed Sequence Tags are the short single pass reads obtained from the ends of a cDNA clone. They are currently the most widely sequenced moiety in case of nucleotide sequences. ESTs provide a cost effective way to gene indexing, gene discovery and the characterization of alternatively spliced forms. The information 01: what gene EST belongs to or is it coming from a new gene can be easily done by clustering the EST sequences and assembling them into contigs, indexing them such that each contig contains the information for only one gene. The present study was aimed at the same principle, following which, 3′-ESTs from Arabidopsis thaliana were clustered and a total of 4018 contigs were generated. Of the total 31,518 ESTs under study, 17,853 remained unassernbled and were marked as unasscmbled or Singleton ESTs. Among the 4018 contigs. 3767 coutigs gave a match with the nonredundant database with a success rate of 93.75%.
Key words: EST, CODONCODE ALIGNER, NCBI, BLAST.
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