Geeta siddhu, gulfam ali and Sarita
Department of Botany, Kisan P.G. College, Simbhaoli-245207, India
email: dr.geetasiddhu@yahoo.in
Abstract: An experiment was conducted to see the impacts of heavy metal stress as it is main cause in soil and water disorders in agricultural field crops, specially Vigna radiata L. cv. MH98-6 on enzyme activity and yield attributes. Surface sterilized seeds of Vigna radiata L. cv. MH98-6 L. were exposed to various concentrations of cadmium chloride solution (10-2 M, 10-4 M, 10-5 M, 10-8 M and control) at room temperature and these seeds were transferred to petriplates and polythene bags in triplicate. Increase in heavy metal stress (10-2 M) conc. was found to have deleterious effects on pollen growth, plant height, phytomass, number of branches, leaf area, chlorophyll contents and yield attiributes while 10-8 M revealed slightly promotory effects. Nitrate and nitrite reductase activity was markedly inhibited at higher conc. and same trend was observed in amylase activity. Low dose of cadmium (10-8 M) did not affect soluble sugar contents of seeds but it induced a significant increase at higher conc (10-2 M). It however, did not affect protein contents of seeds, catalase (CAT) and peroxidase(POD) activity of 15 days old seedlings except at higher concentration.
Keywords: Vigna radiata, Cadmium, enzyme activity, sugar, yield attributes
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