D.S. Chauhan* and D.P. Deswal
Department of Seed Science and Technology
CCS Haryana Agricultural University,Hisar
Email: davender_ chauhan@rediffmail.com
Received-22.11.2017, Revised-20.12.2017
Abstract: Study was conducted to compare fresh, natural and accelerated seed lots of wheat with germination and vigour index varied from 98.67 to 44.00 and 2960 to 524.92 respectively. Germination loss became more evident in accelerated ageing as compared to fresh and natural aged lot. Total soluble seed protein banding pattern of different aged seed was revealed that there was decline in band intensity, band numbers or disappearance of some bands with ageing. It is more in accelerated aged lot as compared to natural aged seed lot. Thus, seed lot with slight variation in germination or vigor could also be used for varietal characterization by SDS-PAGE to differentiate the cultivars or even for genetic purity testing, but not the seed lots which were severely aged that lost threshold limit of 50 per cent.
Keywords: Accelerated ageing, Protein profiles, SDS-PAGE, Wheat seed
REFERENCES
Tanksely, S.D. and Jones, R.A. (1981). Application of alcohol dehydrogenase allozymes in testing the genetic purity of F1 hybrids of tomato. Hort. Sci.,16 : 179-181.
Cooke, R.J. (1998). Electrophoresis in plant testing and breeding. In: advances in Electrophoresis, vol. (Eds. A. Charmbach, M. J. Dunn and B.J. Radola) pp: 171-261, VCH Weinheim, New York.
Meng, X.D., Wei, Y.Y., Zhang, M. H. and Li, J.R. (1998). Identification of wax gourd and chich-qua cultivars using RAPD markers. Acta Agricultural Shanghai, 12: 45-49.
Lucchese, C., Dinelli, G., Miggiano, A. and Lovato (1999). Identification of pepper (Capsicum spp.) cultivars by field and electrophoresis tests. Seed Sci. & Technol., 27: 37-47.
Vishwanath, K., Prasanna K.P.R., Gowda R., Rajendra Pradsad S., Narayanaswammy S. and Pallavi, H.M. (2007). Influence of accelerated ageing on total soluble seed protein & profiles of tomato. Seed research, vol. 35(2) : 194-197.
ISTA (1996). International Rules for Seed Testing. (Supliment) Seed Sci. & Technol., 27: 1-135.
Abdul-Baki, A.A. and Anderson, J.D. (1973). Vigor determination in soybean seed by multiply criteria. Crop Sci., 13: 630-633.
Dadlani, M. and Vaerier, A. (1993). Electrophoresis of variety Identification. Technical Bulletin, Division of Seed Science & Technology, IARI, New Delhi.
Anderson, J.D. (1973). Metabolic changes associated with senescence. Seed Sci. & Technol. 1: 401- 416.
Roberts, B.E. and Osborn D.J. (1973). Protein synthesis and viability in rye grains. Seed Ecology (ed) W. Heydecker. Butterworths, London, pp 94-114
Bedi, S., Kaur, R., Sital, S. and Kaur, J. (2006). Artificial ageing 0f Brassica seeds maturity levels. Seed Sci. & Technol., 34: 287-296.
Singh, C.B., Kanujia, V.P., Khan, A.A. and Sachan, C.P. (2002). Effect of accelerated ageing on biochemical composition of rice (Oryza sativa L.) seeds. Seed Tech News, 32: 187.
Abdul- Baki, A.A. and Anderson, J.D. (1972). Physiological and biochemical deterioration of seeds. In: T. KOZLOWSKI ed., Seed Biology, Vol. II. Academic Press, New york.
Galleschi, L., Spano C., Bedi G., Floris, C., Saviozzi, F. and Capocchi, A. (1988). Proteolytic activities in x Haynaldoticum sarodum seeds of different ages. Annals of Botany, 61: 93-97.
Cheng, S.H. and Kao, C.H. (1984). The role of protolytic enzymes in protein degradation during senescence of rice leaves. Physiology Planetarium, 62: 231-237.
Freitas, A.S., Dias, D.C.F.S, Oliverira M.G.A, Dias, L.S.A. and Jose (2006). Physiological and biochemical changes in naturally and artificially aged cotton seeds. Seed Sci. & Technol., 34: 253-264.
Krishnasamy, V. and Yugasandhya, P. (2002). Varietal identification through electrophoresis in maize. Seed Tech News, 32(1): 126.
Sammour, R.H. (1989). Effect of ageing on the major reserve molecules and their related enzyme in natural aged seeds of flax, J. Islamic Acad. Sci., 2(4): 247-251.
Coello and Romos, J.M.V. (1996). Maize DNA polymerase 2 (an alpha type enzyme) suffers major damage after seed deterioration. Seed Sci. Res., 6: 1-7.
Sundareswaran, S. and Krishnasamy, V. (2006). Changes in enzyme activities during seed senescence in pearl millet ( Pennisetum glaucum ). Proceedings of XII National Seed Seminar, p. 185.