2014, Issue 1, Volume 6

ANTIBACTERIAL ACTIVITY OF MARINE MACRO ALGAE HYPNEA CERVICORNIS (RHODOPHYCEAE) COLLECTED FROM CHAGKUMUGAM COASTAL REGION

E. Shiney, Reginald and J. Irene Wilsy

Department of Botany and Research Centre, Scott Christian College, Nagercoil-3

Abstract: To evaluate the antibacterial activity of Organic solvent extracts from marine macro algae Hypnea cervicornis (Rhodophyceae) against the eight pathogenic bacterial strains. The antibacterial activities of methanol, ethyl acetate and aqueous extracts were tested against various organisms Eschercia coli, Staphylococcus aureus, Proteus vulgaris, Klebsiella sps, Pseudomonous aeruginosa, Spreptococcus sps, Enterobacter sp and Neisseria sp.  by using disk diffusion method. The highest antibacterial activity (15.13±0.15mm) was showed by the methanol extract of Hypnea cervicornis against Streptococcus sp and the lowest activity (7.23±0.25mm) was observed in the methanol extract of Hypnea cervicornis against Proteus vulgaris.  The aqueous extract of Hypnea cervicornis was resistant to all bacterial strains.

Keywords: Marine macro algae, antibacterial activity and algal extracts

REFERENCES

Adaikalaraj, G., Raja, D., Johnson, M., Janakiraman, N. and Babu, A. (2012). Antibacterial potential of selected red seaweeds from Manapad coastal areas, Thoothukudi, Tamil Nadu, India. Asian Pacific Journal of Tropical Biomedicine, s1077-s1080.

Balish, E. and Warner, T. (2002). Enterococcus faecalis Induces Inflammatory Bowel Disease in Interleukin -10 Knockout Mice. Am. J. Pathol., 160 (6): 2253-2257.

Bauer, A.W., Kirby, W.M., Sherris, J.C., Turck, M. (1966). Antibiotic suaceptibility testing by a standardized single disk method. Am. J.Clin. Pathol. 45:493-96.

Becerro, M.A., Lopez, N.I., Turon, X., Uniz, M.J. (1994). Antimicrobial activity and surface bacterial film in marine sponges. J. Exp. Mar. Biol. Ecol.179, 195- 205.

Cordeiro, R. A., Gomes, V.M., Carvalho, A.F.U, and Melo, V.M.M. (2006). Effect of Proteins from the Red Seaweed Hypnea musciformis (Wulfen) Lamouroux on the Growth of human Pathogen yeasts. Brazilian Arch. Boil. Technol, 49(6): 915-921.

Dash, G.K. and Murthy, P.N. (2011). Antimicrobial activity of few selected medicinal plants. International Research Journal of Pharmacy, 2 (1): 146-152.

Emmanuel Joshua Jebasingh, S., Rosemary, S., Elaiyaraja, S., Sivaraman, K., Lakshmikandan, M., Murugan, A. and Raja, P. (2011). Potential Antibacterial activity of Selected Green and Red seaweeds. J.J. JPBMS, 5(14).         

Jawetz, E., Mellnick, J.L. and Adelberg, E.A. (1995). Review of Medical Microbiol, 20th Edition. Applellation Lange Norwalk, Connecticut, pp. 139-218.

Kandhasamy, M and Arunachalam, K.D. (2008). Evolution of Invitro Antibacterial Property of Seaweeds of Southeast Coast of India. African J. Biotech, 7(12): 1958-1961.

Kapil, A. (2005). The challenge of antibiotic resistance: Need to contemplate. Indian J. Med. Res., 121:83-91.

Kolanjinathan, K and Stella, D. (2009). Antibacterial activity of Marine Macro algae against human pathogens. Recent Res. Sci. Techno, 1 (1): 20-22.

Madhusudan, C.S., Manoj, K., Rahul, and C.M. Rishi. (2011). Seaweeds: a diet with nutritional, medicinal and industrial value. Res. J.Med. Plant. 5:153-157.

Mathias, A.J., Somashekar, R.K., Sumithraand, S. and Subramanya, S. (2000). An Assessment of Reservoirs of Mullti-resistant Nosocomial Pathogens in a Secondary care hospital. Indian J. Microbial., 40:183-190.

Mohamed Elanwar H.Osman, Atef M. Abushady and Mostafa E.Elshobary. (2010). In vitro screening of antimicrobial activity of extracts of some macro algae collected from Abu-Qir bay Alexandria, Egypt. African Journal of Biotechnology Vol. 9(12), pp. 7203-7208.                 

Nakajima, K., Yokoyama, A. and Nakajima, Y. (2009). Anticancer effects of a tertiary sulfonium compound, dimethylsulfoniopropionate, in green sea algae on Ehrlich ascites carcinoma-bearing mice. J.Nutr. Sci. Vitaminol. 55: 434-438.

Naqvi, S.W. A., Solimabi, S.Y., Kamat, L., Fernandes, C.V.G., Reddy, D.S. Bhakuni and B.N. Dhawan. (1981). Screening of some marine plants from the India coast for biological activity. Bot. Mar., 24:51-55.

Prasanna Latha, D. and Hema Latha, K.P.J. (2011). Antibacterial activity of the chloroform extracts of the chlorophycean seaweeds Enteromorpha compressa and Chaetomorpha antennina. Int. Re. Jour. Microbio. Vol. 2(8) pp. 249-252.

Priyadharshini, S., Bragadeeswaran, S., Prabhu, K. and Sophia Rani, S. (2012). Antimicrobial and hemolytic activity of seaweed extracts Ulva fasciata (Delile 1813) from Mandapam, Southeast coast of India. Asian Pacific Journal of Tropical Biomedicine, s38-s39.

Rangaiah,S.G., Lakshmi, P and Manjula, E. (2010). Antibacterial activity of Seaweeds Gracilaria, Padina and Sargassum sps on clinical and phytopathogens. Int. J. Chem. Anal. Sci, 1(6): 114-117.

Salem, W.M., Galal, H. and Nasr El-deen, F. (2011). Screening for antibacterial activities in some marine algae from the red sea [Hurghada, Egypt]. African Journal of Microbiology Research Vol. 5(15) pp.2160-2167.

Singh, S.K., Panday, V.D., Singh, A. and Singh, C. (2009). Antibacterial activity of seed extracts of Argemone Mexicana L. on some pathogenic bacterial strains. CEMS, IMS, Baranas Hindu University.

Sorum, H. andT.M.L.  Abee- Lund. (2002). Antibiotic resistance in food- related bacteria- aresult of interfering with the global web of bacterial genetics. Intl. J. Food. Microbial. 78:43-56.

Taylor, P.C., Schoenknecht, F.D., Sherris, J.C., Linner, E.C. (1983). Determination of minimum bactericidal concentrations of oxicillin for Staphylococcus aureus: influence and significance of technical factors. Antimicrob. Agents Ch., 23 (1):142-150.

Tuney, I., Cadirci., B.H., Unal, D. and Sukatar, A. (2006). Antimicrobial activities of the extracts of marine algae from the coast of Urla (Izmir, Turkey). Turk. J.Biol., 30:171-175.

Wijnands, L. M. (2008). Bacillus cereus associated food borne disease: quantitative aspects of exposure assessment and hazard characterization. PhD Thesis, Wageningen University, Wageningen, Netherlands, pp. 1-175.

Zemke- White, W. L. and M. Ohno. (1999). World seaweed utilization: an end-of- century summary. J. Appl. Phycol. 11:369-376.