Prafull Kumar1, Avadhesh Kumar Koshal2 , Sanjay Kumar3 and Manoj Kumar Sharma3
1Department of Genetics and Plant breeding, C.S. Azad University of Agriculture and Technology, Kanpur-208 002, (Uttar Pradesh) India
2Project Directorate for Farming Systems Research (P.D.F.S.R.), Modipuram, Meerut (Uttar Pradesh) India
3 Sanjay Kumar, Janta Vedic College, Baraut, Baghpat (Uttar Pradesh) India
Abstract: Lentil (Lens culinaris Medic) is an important pulse crop in India with advancement and development of hundreds of varieties and introduction of intellectual property rights it is necessary to identifying them individually for identification and registration purposes. The present investigation was carried out during 2012-2013 in biotechnology lab, Department of genetics & Plant Breeding, C.S. Azad university of agriculture and technology, Kanpur with 14 genotypes of Lentil PL-4, KLS-218, KLS-320, L4147, K-75, KLB-08-4, KLS-09-3, VL-126, JL-1, L84-8, PL-5, KLB-303, IPL-81, DPL-62 for protein profiling through SDS-PAGE.
In present investigation, 14 variety of Lentil were studied for varietal identification through electrophoresis. Protein was extracted from dry seed of lentil varieties and analysed by SDS-PAGE . On the basis of photographs, electrophoregrams, Rm values and dendograms (UPGMA cluster analysis) of banding patterns through SDS-PAGE, results found that the number of protein bands found in 14 genotypes ranged from 12 to 20 with Rm value 0.07 to 0.93 for tris soluble proteins. Protein banding pattern of tris soluble proteins was found more distinct in SDS-PAGE. In UPGMA cluster analysis all the genotypes fall in seven cluster groups. SDS-PAGE for tris soluble proteins found suitable for testing distinctness, uniformity, stability of varieties for registration and identification.
On the basis of results, this can be said for characterization and identification of genotypes of lentil, that electrophoretic profile for tris soluble proteins through SDS-PAGE was resulted distinct banding pattern and act as ‘genotypic finger printing’. Therefore, electrophoregram of tris soluble protein in SDS-PAGE was found much better for identification of genotypes in lentil.
Keywords: Lentil, SDS-PAGE, Varietal identification, UPGMA
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