Vikas Sharma1*, Navneet Kour1, Shashank K Singh2, Prasoon Gupta2 andShivangi Sharma3
1Natural Products Laboratory, Division of Biochemistry, Faculty of Basic Sciences, Sher-e-Kashmir University of Agricultural Sciences and Technology of Jammu, Main Campus Chatha,
Jammu-180 009, J&K, India
2Cancer Pharmacology Division and Natural Product Chemistry Division,
Indian Institute of Integrative Medicine, Canal Road Jammu-180001, J&K, India
3 Department of Chemistry,Govt. Degree College, RS Pura -181102, Jammu,
Jammu & Kashmir, India
Email: vikas.skuast@gmail.com
Received-28.03.2025, Revised-12.04.2025, Accepted-25.04.2025
Abstract: The present research work aims to investigate the in vitro cytotoxic potential of Grewia asiatica (phalsa) fruit against human cancer cell lines and further isolation of active ingredient. The methanolic extract was prepared and further fractionated with hexane, chloroform and butanol in the order of increasing polarity. The extract and fractions were evaluated against eight human cancer cell lines (A-549, HCT-116, HT-29, SW-620, PC-3, MCF-7, MDAMB-231, MIAPACA) from five different tissues (lung, colon, prostate, breast, pancreatic) respectively at the conc. of 100 µg/ml via SRB assay. The results revealed that chloroform fraction of G. asiatica exhibited in vitro cytotoxicity against colon cancer cell line (SW-620) with growth inhibition of 70% whereas hexane fraction also inhibited the growth of same cell line by 76%. A compound namely 5-hydroxy methyl furfural (5-HMF) was isolated from chloroform fraction through column chromatography and characterized via NMR (1H and 13C) and mass spectroscopy (HRMS). It was tested against SW-620 cancer cell line and the IC50 value was calculated that showed growth inhibitory potential of the component against colon cancer cells.
Keywords: Grewia asiatica, 5-HMF, SRB assay, In vitro cytotoxicity, Cancer cell lines
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