2025, Issue 7, Volume 17

TOXICITY OF INSECTICIDES AGAINST HONEY BEES IN LABORATORY CONDITION

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G.P. Painkra*

Indira Gandhi Krishi Vishwavidyalaya, College of Agriculture and Research Station, Shankargarh- 497118, Distt-Balrampur- Ramanujganj (C.G.) India

Email: gppainkrarmd@gmail.com

Received-24.06.2025, Revised-10.07.2025, Accepted-24.07.2025

Abstract: Insecticides are harmful for all living organism. Many insecticides are harmful to honeybees, with neonicotinoids and some acaricides being particularly harmful, even at low dose, causing mortality, behavioral issues and weakened immune system. On the basis of toxicity, they are categorized in three groups i.e. highly toxic, moderately and nontoxic to honeybees. Toxic insecticides do not used during the flowering of the crop because bees died in the field and also died inside and outside of the hives after coming from the insecticides applied field. Insecticides should be applied during the evening hour or migrate the bee colony for safety of the bees. It has been observed that after 8 to 10 days of application of insecticides Apis cerana indica colonies were found mortality but Apis mellifera colonies were not observed.

Keywords: Apis cerana indica, Apis mellifera, stingless bee, bumble bee, Honey bee

REFERENCES

Akca, I., Tuncer, C., Guler, A. and Saruhan, I. (2009a). Residual toxicity of eight different insecticides on honey bee (Apis mellifera, Hymenoptera: Apidae). J. Animal Vet. Advances, 8(3): 436-440.

Google Scholar

Akca, I., Tuncer, C., Guler, A. and Saruhan, I. (2009b). Determination of lethal concentration of some insecticides to honey bee Apis mellifera (Apidae, Hymenoptera) with laboratory biossays.  J. Aninmal Vet. Advances, 8(11): 2380-2382.

Google Scholar

Aliouane, Y., Hassani, A.k.EL., Gary, V., Armengaud, C., Lambin, M. and Gauthier M. (2009). Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environmental Toxicology and Chemistry, 28(1): 113-122.

Google Scholar

Anderson, L. D. and Atkins J. E. L. (1958). Toxicity of pesticides to honey bees in laboratory and field tests in South California, J. Economic Ento., 51(1): 103-108.

Google Scholar

Arzone, A. and Patetta, A. (1989). Research on the action of azinphos- methyl, diazinon, dithianon, hexythiazox, omethoate and propargite on honeybees. Apicoltore-Moderno, 80(6):253-261.

Google Scholar

Atallah, M.A., Zeitoun, Z.A. and Hassan, A.R. (1988). The relative toxicity of some synthetic pyrethroid and carbamate insecticides on worker honeybees (Apis mellifera L.) in the laboratory. Proceedings of the Fourth International Conference on Apiculture in Tropical Climates, Cairo, Egypt, 6-10 Nov.:214-217.

Google Scholar

Bai, A.R.K. and Reddy, C.C. (1977). Laboratory toxicity of some insecticides to Apis cerana indica. J. Apicul. Res., 16(3):161-162.

Google Scholar

Bhatt, A.A. (1987). Relative toxicity of some insecticides to adult of the beetle, Adoretus spp. and to the honeybee, Apis cerana indica Fabr. Ind.  J. Plant Protection, 15(1): 97-99.

Google Scholar

Uchler, R., and Drescher, W. (1989). Contact toxic effect of Thiodan on the honey bee, Apis mellifera L., in relation to various surfaces. J. Applied Entomology, 108(3):227-233.

Google Scholar

Choi, S.Y., Kim, Y.S., Lee, M.L., Oh, H.W. and Jeong, B.K. (1989). Studies on the acute and chronic toxicities of pesticides to honeybees, Apis mellifera. Korean-J. Apiculture,4(2):85-95.

Google Scholar

Clinch, P.G. (1967). The residual contact toxicity to honeybees of insecticides sprayed on to white clover (Trifolium repens L.) in the laboratory. New Zealand J. Agril. Res., 10(2): 289-300.

Google Scholar

Danka, R.G., Rinderer, T.E., Hellmich,II, R.L. and Collins, A.M. (1986). Comparative toxicities of four topically applied insecticides to Africanized and European honeybee (Hymenoptera: Apidae). J. Econ. Entomol., 79:18-21.

Google Scholar

Deshmukh, P.B., Mathai, A.T. and Pillai, K.S. (1989). Preliminary laboratory test on the efficacy of a new pyrethroid insecticide cyhalothrin. Pestology, 13(10):15-17.

Google Scholar

Dhanasekaran, S. (2007). Comparative toxicity of combination insecticides to the honeybee, Apis cerana indica Fab. Hexapoda, 14(1):35-37.

Google Scholar

Fiedler, L. (1987). Assessment of chronic toxicity of selected insecticides to honeybees. J. Apicul. Res., 26(2): 115-122.

Google Scholar

Gour, I.S. and Pareek, B. L. (2005). Relative toxicity of some insecticides to coccinellid, Coccinella septempunctata Linn. and Indian honey bee, Apis cerana indica. Indian J. Agric. Res., 39(4): 299-302.

Google Scholar

Gough, H.J., McIndo, E.C. and Lewis,G.B. (1994). The use of dimethoate as a reference compound in laboratory acute toxicity tests on honey bees (Apis mellifera Lib). J. Apicul. Res., 33(2):119-125.

Google Scholar

Gromisz, M. and Gromisz, Z. (1996). Laboratory investigations on the sensitivity of bees to harmful effects of the Bulldock formula. Pszczelnicze- Zeszyty- Naukowe (Poland). 40(1):161-166.

Google Scholar

Gromisz, M. and Gromisz, Z. (1997a). Laboratory testing of the harmful effects of Sanmite in bees. Pszczelnicze- Zeszyty- Naukowe (Poland). 40:151-158.

Google Scholar

Gromisz, M. and Gromisz, Z. (1997b). Laboratory testing of the harmful effects of Karate 025 EC in bees. Pszczelnicze- Zeszyty- Naukowe (Poland). 41:113-119.

Google Scholar

Gromisz, M. and Gromisz, Z. (1998). Laboratory evaluation of the toxicity of the agent Aztec in bees. Pszczelnicze- Zeszyty- Naukowe (Poland). 42(1):185-190.

Google Scholar

Hagler, J. R., Waller, G.D. and Lewis, B.E. (1989). Mortality of honeybees (Hymenoptera: Apidae) exposed to permethrin and combinations of permethrin with piperonyl butoxide. J. Apicul. Res., 28(4):208-211.

Google Scholar

Iwasa, T., Motoyama, N., Ambrose, J.T. and Roe, R.M. (2004). Mechanism for the differential toxicity of neonicotinoid insecticides in the honey bee, Apis mellifera. Crop protection, 23:371-378.

Google Scholar

Kakamand, F.A.K., Mahmoud, T.T. and Amin, A.B.M. (2008). The role of three insecticides in disturbance the midgut tissue in honey bee Apis mellifera L. workers. J. Dohuk Univ., 11(1):144-151.

Google Scholar

Kumar, J., Patyal, S.K. and Mishra, R.C. (1986). Evaluation of some essential oils as repellents to the Indian honey bee Apis cerana indica 1. Gustatory repellency and toxicity. J. Apicul. Res., 25 (4): 256-261.

Google Scholar

Kumar, V., Kumaran, B., Boomathi, N., Saravanan, N.P.A. and Kuttalam, S. (2010). Toxicity of spirotetramat 150 OD to honeybees. Madras Agril. J., 97(1/3):    86-87.

Google Scholar

Laurino, D., Porporato, M., Patetta, A. and Manino, A. (2011). Toxicity of neonicotinoid insecticides to honey bees: laboratory tests. Bulletin of Insectology, 64(1): 107-113.

Google Scholar

Lingappa, S., Jagadish, A., Shivaramu, K. and Prabhuswamy H.P. (1985). Relative intrinsic toxicity of seven insecticides to foragers of the Indian hive bee, Apis cerana indica F. International J. Tropical Insect Sci.,6: 567:568.

Google Scholar

Macieira, O. J. D. and Beraldo, M. J. A. H. (1989). Laboratory toxicity of insecticides to workers of Trigona spinipes F. (Hymenoptera, Apidae). J. Apicul. Res., 28(1):3-6.

Google Scholar

Marletto, F., Patetta, A. and Manino, A. (2003). Laboratory assessment of pesticide toxicity to bumblebees. Bulletin of Insectology, 56(1): 155-158.

Google Scholar

Mayer, D. F. and Lunden, J.D. (1999). Field and laboratory tests of the effects of fipronil on adult female bees of Apis mellifera, Megachile rotundata and Nomia melanderi. J. Apicul. Res., 38(3-4):191-197.

Google Scholar

Murray, A. (1985). Acute and residual toxicity of a new pyrathroid insecticide, WL85871, to honeybees. Bulletin-of- Environl.-Contamin. Toxicol., 34(4): 560-564.

Google Scholar

Muranjan, P.N., Gandhale, D.N., Chaudhari, C.S., Patil, B.D., Pokharkar, D.S. and Naik, R.L. (2006). Toxicity of ready-mix formulations of Pyrethroids and ad-mixed insecticides on forager honey bee, Apis cerana indica Fab. Annals Plant Protecn. Sci., 14(1): 90-93.

Google Scholar

Painkra, G.P., Shrivastava, S.K., Shaw, S.S. and Gupta, R. (2015).Contact toxicity of some commonly used insecticides and new molecules as per recommended dose for crop pests against Indian honey bee, Apis cerana indica Fabr. in laboratory condition.Progressive Research –An International Journal, 10(3):301-302.

Google Scholar

Painkra, G.P., Shrivastava, S.K., Shaw, S.S. and Gupta, R. (2016).Contact toxicity of some commonly used insecticides and new molecules as per recommended dose for crop pests against Indian honey bee, Apis cerana indica Fabr. in laboratory condition. Eco. Env. & Cons., 22 (1): 455-458.

Google Scholar

Panda, P., Sontakke, B.K., Swain, L.K. and Mohapatra, R.N. (1989). Laboratory studies on the contact toxicity of some insecticides against Apis cerana indica Fabr. Ind. Bee J., 51(2):50-52.

Google Scholar

Pastagia, J. J. and Patel, M. B. (2007a). Relative contact toxicity of some insecticides to worker bees of Apis cerana F. J. Plant Protecn. Environt., 4(2): 89-92.

Google Scholar

Rajeswaran, J., Santharam, G., Chandrasekaran, S. and Jayakumar, R. (2003). Contact toxicity of carbosulfan to Apis cerana indica Fab. Indian Bee J., 65 (1&2): 44-47.

Google Scholar

Reddy, M. L. K. (1997). Relative toxicity of some insecticides to honey bees (Apis cerana indica Fab.). J. Res. ANGRAU., 25(4):26-28.

Google Scholar

Sharma, D. and Abrol, D. P. (2005). Contact toxicity of some insecticides to honeybee Apis mellifera (L.) and Apis cerana (F).  J.  Asia-Pacific Entomol., 8(1):113-115.

Google Scholar

Shelke, M. S., Bhalkare, SK, Satpute, NS, Shinde, MP, Tarale, SD and Ubarhande, PP. (2024).Contact toxicity of insecticides against honeybees, Apis mellifera L. under laboratory conditions.International Journal of Advanced Biochemistry Research. SP-8(10): 1074-1079.

Google Scholar

Sim, Y.G., Choi, Y.E., Sim,Y.G. and Choi, Y.E. (1993). Influence of honeybee pollination on soybean yield and yield components. Korean J. Applied Ento., 32(3): 271-278.

Google Scholar

Singh, M.T., Sharma, P.L. and Dhaliwal, H.S. (1974). Toxicity of insecticides workers, Apis cerana indica to honey bee. Pesticides, 8(12): 28-29.

Google Scholar

Singh, P.B., Singh, D.S., Naim, M. and Srivastava, V.S. (1997).  Contact toxicity of some insecticides to oriental honeybee, Apis cerana indica forager. Annals Plant protection Sci., 5(2):207-209.

Google Scholar

Singh, DK. (2012). Pesticide chemistry and toxicology. Bentham Science Publisher. pp. 50-61.

Google Scholar

Singh, J., Schädler, M., Demetrio, W., Brown, G.G. and Eisenhauer, N. (2019). Climate change effects on earthworms – a review. Soil Org. 91: 114-138. doi: 10.25674/so91iss3pp114.

Google Scholar

Tasei, J.N., Lerin, J. and Ripault, G. (2000). Sub-lethal effects of imidacloprid on bumblebees, Bombus terrestris (Hymenoptera: Apidae), during a laboratory feeding test. Pest-Management-Sci., 56(9): 784-788.

Google Scholar

Wael, L.D. and Laere, O.V. (1989). Toxicity and the repellent activity of synthetic pyrethriods towards the honeybee (Apis mellifera L.). Proceedings of the XXXIst International Congress of Apiculture, Warsaw, Poland, Aug. 19-25:209-216.

Google Scholar

Way, M.J. and Synge, A.D. (1948). The effects of DDT and of Benzene Hexachloride on bees. Annals of Applied Biology, 35(1):94-109.

Google Scholar

Weaver, N. (1950). Toxicity of organic insecticides to honeybees: stomach poison and field tests. J. Economic Entomolpgy, 43:333-337.

Google Scholar

Wilkaniec, Z. (1980). The toxicity of some pesticides to honeybees in laboratory investigations. Roczniki Akademii Rolniczej w Poznaniu. 120:147-151.

Google Scholar