Banda Sailatha1, Shalini Mudalkar1*, Sreedhar Bodiga2, Reeja Sundaram1, Akkenapally Snehanjali1 and Eega Kavya Sree1
1Dept. of Forest Biology and Tree Improvement, Forest College and Research Institute,
Mulugu, Siddipet, Telangana
2Dept. of Basic and Social Sciences, Forest College and Research Institute, Mulugu,
Siddipet, Telangana
3Dept. of Silviculture and Agroforestry, Forest College and Research Institute, Mulugu,
Siddipet, Telangana
Email: shalini.mudalkar@gmail.com
Received-03.11.2022, Revised-14.11.2022, Accepted-25.11.2022
Abstract: The present research entitled “Standardization of micropropagation technique for in vitro callus generation from nodal explants of Buchananiacochinchinensis (Lour.) Almeida.” was conducted in the tissue culture laboratory of Forest Biology and Tree Improvement, Forest College and Research Institute, Mulugu, Siddipet during 2020-22. The main objective of the present study was to generate in vitro callus from the nodal explants of Buchananiacochinchinensis. Callus was generated from the cut end of nodal explants after 3-4 weeks of inoculation. Maximum callus response (178.00 ± 2.08) was observed in WPM supplemented with 2mg/L TDZ and optimum callus response (155.66 ± 0.88) was observed in MS supplemented with 2,4-D (4mg/L) and followed by MS with 3mg/L 2,4-D and minimum callus response of 34.66 ± 2.60 was observed in MS alone and followed by MS supplemented with 0.5mg/L 2,4-D. In the present study, the protocol was standardized for callus generation from the nodal explants.
Keywords: Callus, Chironji, In vitro, Nodal explant, Murashige & Skoog, Woody Plant Medium
REFERENCES
Avani, P., Bauri, F.K. and Sarkar, S.K. (2015). Chironji: a golden nut fruit of Indian tribes. In III International Symposium on Underutilized Plant Species, 1241: (pp. 37-42).
Behera, K.K., Mishra, N.M., Dhal, N.K. and Rout, N.C. (2008). Wild edible plants of Mayurbhanj district, Orissa, India. J. Econ. Taxon. Bot, 32; 305-314. J. Econ. Taxon. Bot., 32: 305-314.
Chauhan, P.S., Jitendra, S. and Kavita, A. (2012). Chironjee: a promising tree fruits of dry subtropics. HortFlora Research Spectrum, 1(4): 375-379.
Das, P., Samantaray, S. and Rout, G.R. (1996). In vitro propagation of Acacia catechu, a xerophilous tree, Plant Tiss. Cult., 6 (2): 117/126.
Davis, E.D. (2022). Study on the Best Surface Sterilization Method of Acacia. Journal of Agriculture and Environmental Science. 2(1): 26-31.
Gomez, K.A. and Gomez, A.A. (1984). Statistical Procedures for Agricultural Research. 2nd, John Wiley and Sons USA.
Malik, S.K., Chaudhury, R., Panwar, N.S., Dhariwal, O.P., Choudhary, R. and Kumar, S. (2012). Genetic resources of Chironji (BuchananialanzanSpreng.): a socio-economically important tree species of central Indian tribal population. Genetic resources and crop evolution, 59(4): 615-623.
Meshram, P.B. and Soni, K.K. (2014). Insect pests and diseases of Buchananialanzan and their management in central India. Indian Journal of Tropical Biodiversity, 22(1); 28-38.
Minipara, D., Dhaduk, H., Patil, G., Narayanan, S. and Kumar, S. (2019). Identification of best surface sterilization treatment and control of endophytic bacterial contamination in Annona squamosa L. Int. J. Plant Soil Sci., 29; 1-10.
Nathar, V.N., Ghulam, M.Y., and Bondre, V.N. (2015). Callogenesis in Semecarpusanacardium L.-Anti cancerous medicinal plant. International Journal of Advanced Life Sciences, IJALS, 8(1): pp.90-96.
Ndoye, M., Diallo, I. and Gassama, Y.K. (2003). In vitro multiplication of the semi-arid forest tree, Balanitesaegyptiaca (L.) Del. African Journal of Biotechnology, 2(11): 421-424.
Patel, I. and Raipur, I.G.K.V. (2018). Standardization of in vitro propagation protocol for chironji(Buchananialanzanspreng).
Rajeswari, V. and Paliwal, K. (2008). In vitro adventitious shoot organogenesis and plant regeneration from seedling explants of AlbiziaodoratissimaLf (Benth.). In Vitro Cellular & Developmental Biology-Plant, 44(2): 78-83.
Rajput, B.S., Gupta, D., Kumar, S., Singh, K. and Tiwari, C. (2018). BuchananialanzanSpreng (Chironji): A vulnerable multipurpose tree species in Vindhyan region. Journal of Pharmacognosy and Phytochemistry, 7(5): 833-836.
Shields, R., Robinson, J.S. and Anslow, A.P. (1984). Use of fungicides in plant tissue cultures. Plant Cell Rep., 3: 33-36.
Singh, C.K., Raj, S.R., Patil, V.R., Jaiswal, P.S. and Subhash, N. (2013). Plant regeneration from leaf explants of mature sandalwood (Santalum album L.) trees under in vitro conditions. In Vitro Cellular & Developmental Biology-Plant, 49 (2): 216-222.
Singh, J., Patra, A.K., Nandeshwar, D.L., Meshram, P.B. and Negi, K.S. (2002). Effect of growth regulators on the rooting of root cuttings of Chironji (BuchananialanzanSpreng). Proce. of Nat. Workshop. Conserv. of Medic. Plants,128 (6): 11-12.
Singh, S. and Singh, A.K. (2014). Standardization of softwood grafting in Chironji (BuchananialanzanSpreng.) under semi-arid environment of western India. Indian Journal of Horticulture, 71(1): 120-122.
Singh, S., Singh, A.K. and Apparao, V.V. (2006). Genetic diversity in chironji (Buchananialanzan) under semi-arid ecosystem of Gujarat. Indian Journal of Agricultural Sciences, 76(11): 695.
Singh, S., Singh, A.K., Bagle, B.G. and More, T.A. (2010). Chironji: A Potential Dry Fruit for Dry Lands. Technical Bulletin, (33).
Singh, S.V., Sharma, R., Ramakrishnan, R.S., Sharma, S., Dwivedi, N., Kumar, A. and Gaur, V.S. (2022). In vitro mass multiplication of economically endangered forest plant Chironji (BuchananialanzanSpreng.). Indian journal of genetics and plant breeding. 82(01): 116-120.
Siva, S.M., Priya, T.A., Balasubramanian, P., Manimekalai, V. and Ravichandran, P. (2015). In vitro regeneration of an endangered tree-Elaeocarpus blascoi Weibel. (Rudraksha) from Southern Western Ghats, Tamil Nadu, India. In vitro.3(11): 62-66.
Swastika, B. and Abhijit, B. (2015). Buchananialanzanspreng: a veritable storehouse of phytomedicines, Asian journal of pharmaceutical and clinical research, 8 (5):18-22.
Tewari, R.K. and Bajpai, C.K. (2001). Propagation of Chironjee (BuchananialanzanSpreng.) by chip budding. Agrofor.Newsletter. 13(1&2): 1-2.