Vishal Kumar Deshwal1* and Malik Mohd. Muhiuddin Siddiqui 2
1Department of microbiology, Doon (PG) Paramedical College and Hospital, Dehradun, India
2Department of Biotechnology, Singhania University, Pacheri Bari, Jhunjhunu- Rajasthan, India
Email-* vishal_deshwal@rediffmail.com;
Abstract: In present study, aqueous and alcoholic extract of both parental and in vitro medicinal plant Tylophora indica was selected for evaluate antimicrobial activity against Staphylococcus aureus ATCC 25923, Streptococcus agalactiae, Enterococcus faecalis, Staphylococcus epidermidis, Streptococcus pyogenes, Bacillus species. Agar well diffusion was used to evaluate antimicrobial activity. Results indicated antibacterial activity of the aqueous and alcoholic extracts of in vitro raised calli of Tylophora indica against the tested gram-positive bacteria are shown in the table 1. Significant activity (P<0.05) was observed against Staphylococcus aureus and Staphylococcus epidermidis in the alcoholic leaf callus extract. Against these gram positive bacteria no significant activity was exhibited by the aqueous leaf callus extract. No activity wa s observed against the tested gram-positive bacteria in alcoholic as well as aqueous extracts of root and nodal calli.
Keyword: Antimicrobial activity, Tylophora indica
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