Vishal Kumar Deshwal1* and Malik Mohd. Muhiuddin Siddiqui 2
1Department of Microbiology, Doon (PG) Paramedical College and Hospital, Dehradun
2Department of Biotechnology, Doon (PG) Paramedical College and Hospital, Dehradun
Abstract: The results showed that aqueous and alcoholic extracts of leaf of parent plant of Coleus forskohlii showed antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis and Bacillus species. Alcoholic extract of stem of of parent plant of Coleus forskohlii showed significant activity against Staphylococcus aureus and Staphylococcus epidermidis. Antibacterial activity shown by the alcoholic as well as aqueous extracts of in vitro raised plant of Coleus forskohlii against the gram-positive bacteria was tested. The significant amount of activity against Bacillus species only was shown by the alcoholic leaf extract but the alcoholic stem extract exhibited mild but statistically insignificant (P<0.05) activity against Enterococcus faecalis and Bacillus species. The aqueous leaf extract of parent plant of Stevia rebaudiana exhibited significant activity against only Staphylococcus epidermidis andthe alcoholic leaf extract showed significant activity against Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis and Bacillus species. MIC values of the alcoholic leaf extract of parent plant of Coleus forskohlii against the tested gram-positive bacterial species ranged from 9.43×10-3 to 18.65×10-3 µg/ml and MIC values of the aqueous leaf extract of parent plant of Coleus forskohlii against the tested bacterial species ranged from 3.15×10-3 to 4.65×10-3 µg/ml. MIC values of the alcoholic leaf extract of parent plant of Stevia rebaudiana against the tested gram-positive bacterial species ranged from 9.43×10-3 to 25.15×10-3 µg/ml and aqueous leaf extract of parent plant of Stevia rebaudiana against Staphylococcus epidermidis was found to be 12.5×10-3 µg/ml.
Keyword: Antimicrobial activity, MIC, Coleus forskohlii, Stevia rebaudiana
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