P.K. Sharma, M. Kumar, A. Garg*, V. Dhaka and D.K. Sharma**
Department of Microbiology J. V. College, Baraut (Baghpat)-250 611
Abstract: Bacterial contamination of the water supply of newly installed water system unit was investigated. Water samples were collected at different sites from water supply lines to the final utility area. Within hours following connection and continuing for up to several months of the study, water samples were obtained from the air-water syringe of the Unit. The samples were plated on culture media for quantitative analysis. The Total Viable Count procedure involved incubation of sampled water in aerobic conditions and does not allow estimation of anaerobic microbes. Direct pour plate method and subsequently membrane filtration technique was followed to estimate the microbial load. Finally a comparative analysis of microbial load on different steps was made. However the system was found to be useful monitoring the microbial load.
Key words: Microbial load, Total Viable Count, Water system.
REFERENCES
APHA. (1989). Standards Methods for the Examination of Water and Waste Water. 17th edition, American Public Health Association, Washington, D.C.
Gapp, G.; Guyomard, S.; Nabet, P. and Scouvart, J. (1999). Evaluation of the applications of a system for real-time microbial analysis of pharma-ceutical water systems. European Journal of Parenteral Science. 4 : 131–136.
Kawai, M.; Yamaguchi, N. and Nasu, M. (1999). Rapid enumeration of Physiologically active bacteria in purified water used in the pharmaceutical manufacturing process. Journal of Applied Microbiology. 86 : 496–504.
Kulakov, L.A.; McAlister, M.B.; Ogden, K.L.; Larkin, M.J. and O’Hanlon, J.F. (2002). Analysis of bacteria contaminating ultrapure water in industrial systems. Applied and Environmental Microbiology. 68 : 1548–1555.
McFeters, G.A.; Broadaway, S.C.; Pyle, B.H. and Egozy, Y. (1993). Distribution of bacteria within operating laboratory water purification systems. Applied and Environmental Microbiology 59, 1410– 1415.
Norton, C.D. and LeChevallier, M.W. (2000). A pilot study of bacteriological population changes through potable water treatment and distribution. Applied and Environmental Microbiology. 66: 268–276.
Reasoner,D.J.andGeldreich,E.E.(1985).Anewmedium for theenumerationandsubcultureof bacteria from potable water. Applied and Environmental Microbiology. 49 : 1–7.Wallner, G.; Tillmann, D. and Haberer, K. (1999) Evaluation of the chemscan system for rapid microbiological analysis of pharmaceutical water on PDA. Journal of Pharmaceutical Science and Technology.53 : 70–74.