D. Vijay Kumar Naik 1*, B.V. Bhaskara Reddy 2, R. Sarada Jayalaxmi 3, J. Sailaja Rani 1
and S.M. Shareef 2
1 Department of Plant Pathology, Agricultural College, Mahanandi-518502, Andhra Pradesh, India; 2 Department of Plant Pathology, Regional Agricultural Research Station, Tirupati- 517501, Andhra Pradesh, India; 3 Department of Plant Pathology, Agricultural College, Tirupati-517501,
Andhra Pradesh, India
Email: d.v.naik07@gmail.com
Received-17.03.2015, Revised-10.04.2015
Abstract: Phytoplasma was detected in Cleome viscose and Borreria hispida weeds by direct and nested polymerase chain reaction using universal primers P1/P7 and R16F2n/R16R2 specific to 16SrRNA gene of phytoplasma. Running of 1% agarose gel electrophoresis for confirmation of phytoplasma associated with these two weeds.
Keywords: Nested PCR, Cleome viscosa, Borreria hispida, Phytoplasma specific primers, 1% AGE
REFERENCES
Deng, S and Hiruki, C. (1991). Amplification of 16S rRNA genes from culturable and nonculturable Mollicutes. Journal of Microbiological Methods. 14:53 – 61.
Doyle, J. J and Doyle, J. L. (1990). A rapid total DNA preparation procedure for fresh plant tissue. Focus. 12: 13-15.
Gundersen, D. E and Lee, M. I. (1996). Ultrasensitive detection of phytoplasmas by nested PCR assays using two universal primer pairs. Phytopathologia Mediterranea. 35:144-151.
Purcell, A.R (1982). Insect vector relationships with prokaryotic plant pathogens. Annual Review of Phytopathology. 20:397-417.
Waters, H and Hunt, P. (1980). The in vivo three dimensional form of a plant Mycoplasma like organism by the analysis of serial ultrathin sections. Journal of General. Microbiology. 116:111-131.