D. Vijay Kumar Naik 1*, R. Sarada Jayalaxmi Devi2, B.V. Bhaskara Reddy 3, S.M. Shareef3
and A. Ranga Rani2
1 Department of Plant Pathology, Agricultural College, Mahanandi-518502, Andhra Pradesh, India; 2 Department of Plant Pathology, Agricultural College, Tirupati-517501, Andhra Pradesh, India; 3 Department of Plant Pathology, Regional Agricultural Research Station, Tirupati- 517501, Andhra Pradesh, India;
Email d.v.naik07@gmail.com
Received-05.03.2015, Revised-24.03.2015
Abstract: Phytoplasma was detected in Croton bonplandianum weed by direct and nested PCR using universal primers P1/P7 and R16F2n/R16R2 specific to 16SrRNA gene of phytoplasma. Running of 1% agarose gel electrophoresis for confirmation of phytoplasma associated with this weed.
Keywords: Croton bonplandianum, Nested PCR, 1% AGE, Phytoplasma specific primers
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