PHYSIOLOGY AND INDUCTION OF DEFENSE ENZYMES IN MERISTEM DERIVED CASSAVA (MANIHOT ESCULENTA. CRANTZ) PLANTS

N. Rajinimala¹*, K. Elanchezhyan² and Lenin Raja D.³

¹ Department of Plant Pathology, Tamil Nadu Agricultural University, V.O. Chidambaranar Agricultural College and Research Institute, Killikulam

² Department of Agricultural Entomology, Tamil Nadu Agricultural University, V.O. Chidambaranar Agricultural College and Research Institute, Killikulam

³ Department of Natural Resource Management, Horticultural College and Research Institute, TNAU, Periyakulam 625 604, Tamil Nadu, India

Email: rajinimala@tnau.ac.in

Received-17.07.2024, Revised-05.08.2024, Accepted-23.08.2024

Abstract: Physiology and induction of defense enzymes   in meristem derived and Cassava mosaic virus infected cassava plants (cv. MVD1 and cv. H226) were studied. Chlorophyll a, chlorophyll b, total chrolorophyll, total phenol, total sugars and total soluble protein contents were more in meristem derived healthy cassava plants as compared to Cassava mosaic virus infected cassava plants. SDS-PAGE analysis revealed the presence of 35 KDa protein in Cassava mosaic virus infected samples of cv. MVD1 and cv. H226 collected from field which was absent in meristem derived healthy plants. Induction of enzymes viz., PO, PPO, PAL and catalase was more in Cassava mosaic virus infected cassava plants.

Keywords: Cassava mosaic virus, Chlorophyll, Phenol, Protein, Meristem

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