2022, Issue 10, Volume 14

PHYSICO CHEMICAL CHARACTERIZATION OF SPENT WASH GENERATED DURING ALCOHOL PRODUCTION FROM SWEET SORGHUM JUICE AND ITS IMPACT ON GERMINATION AND SOIL MICRO FLORA

Ananthalakshmi Ranganathan1*, Nandhini Singh Parmar2 and Seema Paroha1

1National Sugar Institute, Kanpur, India

2B.N.D College, CSJM University, Kanpur, India.

Email: ananthuknocks@gmail.com

Received-03.10.2022, Revised-14.10.2022, Accepted-26.10.2022

Abstract: The main objective of this research was to characterize the spent wash generated during alcohol production from sweet sorghum juice(SWSSJ)for its PH, BOD, COD, TSS and TDS and to study their effect in different concentration on seed germination of selected crops. The SWSSJ was acidic and having BOD and COD value in the range of 22900 mg/l and 35600mg/l respectively. The TSS and TDS of spent wash was found to be 3700mg/l and 8000mg/l respectively. Five different seeds – Soya bean, Black chick pea, White peas, Green gram and Rajma were used to study the effect of different dilution of spent wash in petri plates viz Control without spent wash, 5%, 10%,15%,20%,50% and 100% spent wash. Based on the observation noted in petri dish study, the pot study was done with 2 seeds – White peas and Black chick pea with Control (without spent wash), 5%, 10%,15% and 20% spent wash dilutions. After 15 days of sowing, the soil of different treated pots was studied for number of bacterial and fungal colonies. From the study, it is concluded that the SWSSJ containing high level of plant nutrients can be made available to the plants with proper dilution for the better growth and development of the crop.

Keywords: Sweet sorghum, Characterization, Germination, Soil micro flora

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