N. Rajinimala1*, K. Elanchezhyan2, S. Kanmani1, D. Lenin Raja3 and J. Sheela1
1 Department of Plant Pathology, Tamil Nadu Agricultural University, V.O. Chidambaranar Agricultural College and Research Institute, Killikulam
2 Department of Agricultural Entomology, Tamil Nadu Agricultural University, V.O. Chidambaranar Agricultural College and Research Institute, Killikulam
3 Department of Natural Resource Management, Horticultural College and Research Institute, TNAU, Periyakulam 625 604, Tamil Nadu, India
Email: rajinimala@tnau.ac.in
Received-03.09.2024, Revised-15.09.2024, Accepted-29.09.2024
Abstract: Out of different solid media tested for the growth of ground root rot pathogen Macrophomina phaseolina, Potato Dextrose Agar medium (PDA) supported maximum mycelial growth followed by Peanut leaflet Oatmeal Agar medium (POMA). Among different pH tested, the maximum growth of the pathogen was observed at pH 7. Maximum mycelial growth of the pathogen was recorded at 35°C followed by 30°C. Ground nut root rot pathogen was molecularly characterized and confirmed as M. phaseolina by amplifying 530 bp size of ITS region using ITS1 and ITS4 primer pair.
Keywords: Root rot, Macrophomina, Groundnut, Pathogen
REFERENCES
Chakraborty, B.N., Chakraborty, U., Saha, A., Dey, P.L. and Sunar, K. (2010). Molecular characterization of Trichoderma viride and Trichoderma harzianum isolated from soils of North Bengal based on rDNA markers and analysis of their PCR-RAPD profiles. Global Journal of Biotechnology & Biochemistry, 5(1):55-61.
Ikkar, R.B. (2012). Studies on mung bean (Vigna radiata) root rot complex. M.Sc., (Ag.) Thesis. Vasantrao Naik Marathwada Krishi Vidyapeeth, Parbhani.
Mathur, S.B. and Cunfer, B.M. (1993). Seed-borne diseases and seed health testing of wheat. Danish Government Institute of Seed Pathology for Developing Countries, Copenhagen, 168.
Mayee, C.D. and Datar, V.V. (1986). Phytopathometry. Technical bulletin 1 (Special bulletin 3), Parbhani, Marathwada Agricultural University.
Purkayastha, S., Kaur, B., Dilbaghi, N. and Chaudhury, A. (2006). Characterization of Macrophomina phaseolina, the charcoal rot pathogen of cluster bean, using conventional techniques and PCR‐based molecular markers. Plant pathology, 55(1):106-116.