2018, Issue 2, Volume 10

MORPHO-PHYSIOLOGICAL AND BIOCHEMICAL CHARACTERIZATION OF WHEAT UNDER THE WATER DEFICIT CONDITIONS

Prafull Kumar*, Manoj Kumar Yadav, R.S. Sengar, Phushpendra Kumar, Mukesh kumar and S.K. Singh2

Department of Agriculture Biotechnology, College of Agriculture, Sardar Vallabhai Patel    University Of Agriculture & Technology, Modipuram, Meerut (U.P.)

2Department of Genetics and Plant Breeding, College of Agriculture, Sardar Vallabhai Patel    University Of Agriculture & Technology, Modipuram, Meerut (U.P.)

Email: prafull8412@gmail.com

Received-04.02.2018, Revised-24.02.2018

Abstract: In present study the 10 wheat genotypes were evaluated for their morphological, physiological and biochemical characters under drought stress. Drought is one of the most important phenomena which limit crop production and yield. Analysis of variance for morpho-physiological and biochemical traits and yield revealed highly significant differences among the entries under irrigated and non irrigated condition. In this study parameter like plant height, leaf length, number of tiller, spike length, spikelets per spike,  seeds per spike, chlorophyll content, RWC, MSI and proline content was recorded. Analysis of the data showed that under water stress condition HD 2733 showed highest no. of tiller (4.37), Spikeletes per spike (17.20) and seeds per spike (21.20). While highest chlorophyll content genotype DBW 71 (34.37). RWC and MSI under the stress condition genotype HD 2733 performance better. Proline accumulation is believed to play adaptive roles in plant stress tolerance. Accumulation of proline has been advocated as a parameter of selection for stress tolerance. Therefore, the objective of the present investigation was to find out suitable morpho-physiological and biochemical traits that could be invariably used for the yield improvement of wheat grown under drought stress condition, responses to drought is essential for a holistic perception of plant resistance mechanisms to water-limited conditions. Crops demonstrate biochemical responses to tackle drought stress. All these parameters were found to greatly affect under imposed drought condition. Almost all the parameters were showed decline under imposed drought condition except proline content which is known as a stress tolerant indicator.

Keywords: Wheat, Morpho-physiological character, Proline, Drought Stress

REFERENCES

Abbasi, T., Premalatha, M., Abbasi, T. N. and Abbasi, S. A. (2014). Wind energy: Increasing         deployment, rising environmental concerns. Renewable and Sustainable Energy   Reviews 31: 270–288

Bayoumi, T. Y., Eid, M. H. and Metwali, E. M. (2008).Application of physiological and biochemical indices as a screening technique for drought tolerance in wheat genotypes. African J. Biotech. 7: 2341-2352.

Bahieldin, A, Hesham, H.T., Eissa, H.F., Saleh, O.M., Ramadan, A.M., Ahmed, I.A., Dyer, W.E., El-Itriby, H.A. and Madkour, M.A. (2005). Field evaluation of transgenic wheat plants stably expressing the HVA1 gene for drought tolerance. Physiol Plant 123:421–427.

Bates, L. S., Waldran, R. P. and Teare, I. D. (1973). Rapid determination of free proline for water stress studies. Plant Soil. 39: 205-208.

Bajji, M., Lutts, S. and Kinet, J. M. (2001).Water deficit affect on solute contribution to osmatic adjustment as a function of leaf ageing in three duration wheat (Triticum duram Desf.) cultivars performing differently in arid conditions. Plant Science. 160:669-681.

Blum, A. (1999).Drought resistance of a doubled-haploid line population of rice in the field.Workshop on Genetic Improvement of Rice for Water Limited Environments. I RRI, Los Baños, Philippines, 1-3.

Chen, C. T., Chen, L. M., Lin, C. and Kao, C. H. (2001).Regulation of proline accumulation in deta shed rice leaves exposed to excess copper. Plant Sci. 160:283–290.

Claussen, W. (2005).Proline as a measure of stress in tomato plants. Plant Sci. 168:241–248.

Dencic, S., Kastori, R., Kobiljski, B. and Duggan, B. (2000). Evaluation of grain yield and its components in wheat cultivars and landraces under near optimal and drought conditions. Euphytica 113 (1):43-52

Heikal, M. M. and Shaddad, M. A. (1982).Alleviation of osmotic stress on seed germination and seeding growth of cotton, pea and wheat by proline. Phyton (Aust) 22:275-287.

Hassanein, R. A., Hassanein, A. A., Haider, A. S. and Hashem, H. A. (2009).Improving salt tolerance of Zea mays L. Plants by presoaking their grains in glycine betaine.Aust J Basic Appl Sci. 3(2): 928-942.

Mationn, M. A., Brown, J. H. and Ferguon, H. (1989). Leaf water potential, relative water content and diffusive resistance as screening techniques for droughtresistance in barley. Agron. J.81:100-105.

Mirbahar, A. A., Markhand, G. S., Mahar, A. R., Abro, S. A. and  Kanhar, N. A. (2009). Effecct of water stress on yield and yield components of wheat (Triticum avestivum L.) Varieties. Pak. J. Bot., 41(3): 1303-1310.

Hanson, H., Borlang, N.E. and Anderson, R.G. (1982). Wheat in the third world. West View Press, Boulder, Colorado.

Hamayun, M., Khan, S.A., Khan, A.L., Shinwari, Z.K., Iqbal, I., Sohn, E-Y., Khan, M.A. and Lee, I.J. (2010). Effect of salt stress on growth attributes and endogenous growth hormones of soybean cultivar Hwang keum kong. Pakistan Journal of Botany, 42(5): 3103-3112.

Sairam, R.K. and Saxena, D.C. (2000). Oxidative stress and antioxidants in wheat genotypes: possible mechanism of water stress tolerance. J. Agro. Crop. Sci.184:55-61.  

Khakwani, A.A., Dennett, M., Munir, M. (2011). Drought tolerance screening of wheat varieties by inducing water stress conditions. Songklanakarin Journal of Science and Technology, 33:135–142.

Khakwani, A.A., Dennett, M., Munir, M., Abid, M. (2012). Growth and yield response of wheat varieties to water stress at booting and anthesis stages of development. Pakistan Journal of Botany, 44:879–886.

Kadam, Sukshala, Shukla, Yogesh, Narayan, Subhash, Chandrakant, Singh and Kiran, Suthar (2017). Screening of Wheat Genotypes (Triticum durum L.) in Response to Drought Stress by Some Physiological and Biochemical Indices. Int. J. Pure App. Biosci. 5 (3): 969-977.

Sibomana, I.C., Aguyoh, J.N. and Opiyo, A.M. (2013). Water stress affects growth and yield of container grown tomato (Lycopersicon esculentum Mill) plants. Global Journal of Biochemistry and Biotechnology 2(4), 461- 466.

Singh, R.P., Rajara, S., Miranda, A., Huerto-Espino, J. and Crossa, J. (2001).Grain yield and other traits of tall and dwarf isolines of modern bread and durum wheats. (In: Z. Bedo and L. Lang, eds). Wheat in Global Envir. 579-584.

Subhani, G.M., Hussain, M., Ahmad, J., Anwar, J. (2011). Response of exotic wheat genotypes to drought stress. Journal of Agricultural Research, 49(3): 293-305.

Shao, H. B., Chu, L. Y., Jaleel, C. A., Manivannan, P., Panneerselvam, R. and M. A. Shao (2009).Understanding water deficit stress-induced changes in the basic metabolism of higher plants-biotechnologically and sustainably improving agriculture and the ecoenvironment in arid regions of the globe. Crit. Rev. Biotechnol. 29: 131-151.

Simane, B. (1993). Durum wheat drought resistance. PhD. Thesis. Wageningen University, The Netherlands.

Mirzaei, A., Naseri, R. and Soleimani, R. (2011). Response of different growth stages of wheat to moisture tension in a semiarid land. World Appl Sci J. 12(1):83-89.

Nyachiro, J.M., Briggs, K.G., Hoddinott, J. and Johnson Flanagan, A.M. (2001). Chlorophyll content, chlorophyll fluorescence and water deficit in spring wheat. Cereal Res. Comm., 29(1-2):135-142.

Solomon, K.F., Labuschangne, M.T. and Bennie, T.P. (2003). Response of Ethiopian durum wheat (Triticum turgidum var durum L.) genotypes to drought stress. South Africa J. Plant Soil, 20: 54-58.

Sokoto, M. B. and Singh A. (2013). Yield and Yield Components of Bread Wheat as Influenced by Water Stress, Sowing Date and Cultivar in Sokoto, Sudan Savannah, Nigeria. American Journal of Plant Sciences, 4: 122-130.

Zhou, G.A., Chang R.Z. and Qiu L.J., (2010). Overexpression of soybean ubiquitin-conjugating enzyme gene GmUBC2 confers enhanced drought and salt tolerance through modulating abiotic stress-responsive gene expression in Arabidopsis. Plant Mol. Biol., 72: 357-369.