Punit Choudhary*, N.B. Singh and Avanish Sharma
Scientist Agroforestry, KVK Jammu, SKUAST-J
Email: pmd.choudhary@gmail.com
Received-02.09.2019, Revised-22.09.2019
Abstract: Microsatellite markers (SSR’s) were found effective in revealing polymorphisms among twenty two different species/clones of Willows. Out of 10 SSR primers only seven primers produced SSR profiles with intense banding pattern and generated a unique set of amplification products. Out of the total 24 scorable bands, 16 showed polymorphism and eight bands exhibited monomorphism with an average of 3.43 bands per primer. A unique band of approximately 100 bp was generated by SB-80 for S. udensis.Jaccard’s similarity correlation coefficient values was highest value (0.98) between two male genotypes of Salix tetrasperma [S. tetrasperma (TFB) and S. tetrasperma (LNM)] and lowest (0.64) between S. udensis and S. nigra, S. udensis and S. tetrasperma (TWE) and between S. matsudana (PN-722) and S. pierotii and 799.The dendrogram exhibited six clear clusters with S. udensis coming out as an outline. SSR primer namely SB-80 produced unique band present only in S. udensis. This information could be used for characterizing particular genotypes.
Keywords: Clones, Microsatellite markers, Willows
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