MacDonaldIdu1*, Frank C. Okeke2 and Benjamin Ogunma Gabriel3
1Phytomedicine Research Group, Department of Plant Biology and Biotechnology, University of Benin, Benin City, PMB 1154, Edo State, Nigeria
2Department of Biological Sciences, University of Abuja, Nigeria
3Department of Science Laboratory Technology, University of Benin, Benin City, PMB 1154,
Edo State, Nigeria
Email: alexthemain076@gmail.com
Received-25.06.2024, Revised-13.07.2024, Accepted-29.07.2024
Abstract: Objectives: The study showed the antimicrobial properties of the stem bark extracts against selected strains of microbes. The antimicrobial study of the stem bark extracts was established using a standard method. The study investigated the microbicidal and static effect of Boswellia sacra stembark extracts against clinical isolates. The results obtained showed that the sixteen clinical isolates obtained, Shigella dysenteriae (10.30%) was most predominant followed by Escherichiacoli (9.30%) while Bacillussubtilis and Vibriocholera were the least occurring bacteria at 2.10% each. Most of the isolates were gram-negative (53%) and the others (47%) were gram-positive. Sugar utilization test revealed that all the isolates (100%) utilized glucose and sucrose.From the sensitivity test of Boswellia sacra stemback extracts on clinical isolates, Staphylococcus epidermidis, Enterococcus faecalis, Alcaligenes faecalis, Streptococcus pyogenes showed the highest zones of inhibition while Vibrio cholerae, Streptococcus mutans and Enterococcus faecalis showed the least zones of inhibition of the fractions used. Ethanol fraction had the highest activity on the test isolates while N-butanol and N-hexane recorded the least. Susceptibility of clinical isolates to standard antibiotics sensitivities of the test bacteria against commercially available standard antibiotics. Staphylococcus epidermidis was the most susceptible while Vibrio cholera was mostly resistant to the drugs. The Zone of inhibition of extracts were compared with that of different standards like ampicillin, ciprofloxacin, norfloxacin, and chloramphenicol for antibacterial activity and nystatin and griseofulvin for antifungal activity. In conclusion, this study adheres to the ethnomedicinal claim that Boswellia sacra scientifically validated of its potency as antimicrobial effect against several micro-organisms.
Keywords: Microbicidal, Static, Boswellia sacra, Stem bark, Clinical isolates
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