2014, Issue 4, Volume 6

ISOLATON, BIOCHEMICAL CHARACTERIZATION AND PREPARATION OF BIOFERTILIZER USING RHIZOBIUM STRAINS (VIGNA MUNGO) FOR FARMERS USE

Nikita Singh, Purushottam1, Akash Tomar, Ravindar Kumar, B.P. Dhyani2,

Lakshman Prasad3 and Shefali Poonia4

1College of Biotechnology, SVPUA&T, Meerut.

2College of Agriculture, SVPUA&T, Meerut

3Division of Chemical, IARI, New-Delhi.

4Deparment of Botany,D.N.College,Meerut.

Abstract: A pot experiment was conducted at Sardar VallabhBhai Patel University of Agriculture & Technology, Meerut (U.P.) to evaluate the effect of Rhizobium as a biofertilizers on different plant parameters related to yield performance of Black gram (Vigna mungo) cv.urd shekhar-2 during the period from March to June 2013. The trial composed of four treatments such as T1=control, T2=DAP, T3=IARI (Urd 10B) and T4=Native strain. Irrespective of treatment differences the black gram plant as a pulse crop showed a lag phase for slow dry matter production in early growth stage that decrease upto harvest. This greater dry matter production eventually partitioned to root length, seed number, seed weight, dry pod weight, number of pods, number of nodules and microbial count. The results revealed that biofertilization perform significant improvement in plant productivity and quality. The maximum germination and increase in plant root length, seed number, seed weight, dry weight, number of pods and microbial count was increased progressively in treatments treated with Rhizobium.

Keywords: Isolation, biofertilizer, Rhizobium

REFERENCES

Adewusi, H.G., Bada, S.O., Ladipo, D.O., and Husain, T. (2008). Nodulation in Millettia thonningii(Schum & Thonn.) Baker; native Rhizobia and seed interaction from southwest Nigeria. Pak. J.17-29. 843-847.

Aftab, A. and Bano, A. Rhizobium and Phosphate Solubilizing Bacteria Improve the Yield and Phosphorus Uptake in Wheat (Triticum aestivum)  International Journal of Agriculture & Biology  ISSN Print: 1560–8530.

Asad, R., Ahmad, A., Farokh,  D., Ghorban, N.M. and Eslam, M. (2008). Influence of plant growth-promoting Rhizobacteria on dry matter accumulation and yield effect  of Chickpea (Cicer arietinum L.) under field conditions. American-Euraisan J. Agric. and Environ. Sci., 3(2): 253-257.

Burns, R.C; Hardy, R.W.F. (1975). Nitrogen Fixation in Bacteria and Higher Plants. Springer Verlag, New York. pp. 26-30.

Chanda, S.V., Joshi, A.K., Vishnav, P.P., Singh, Y.D. (1987). Growth analysis using classical and curve-fitting methods in relation to productivity in pearl millet (Pennisetum americanum L.). J. Agron. Crop Sci. 159: 312-319.

Chowdhury, M.U; Ullah, M.H; Afzal, M.A; Khanam, D; Nabi, S.M. (1998). Growth, nodulation and yield of cowpea as affected by Rhizobium inoculation on hilly region. Bangladesh J. Agric. Res. 23(2): 195-203.

Hoshang, N; Abas, S. and Rahim, N. (2011). Effect of Integrated Application of Bio-fertilizer on Grain Yield, Yield Components  and Associated Traits of Maize Cultivars. American-Eurasian J. Agric. And Environ. Sci., strains with Bradyrhizobium japonicum. 10(2): 271-277.

Luthra, Y.P; Sheoran, I.S; Singh, R. (1983). Ontogenic interaction between photosynthesis and symbiotic nitrogen fixation in pigeon pea. Ann. Appl. Biol. 103: 549-556.

Mahhok, M; Dhingra, L.R. (1951). Effect of inoculation with specific nodule organism on the growth of peas. Proc. Indian Sci. Congr. 38: 170.

Matiru, V.N. and Dakora, F.D. (2004). Potential use of rhizobial bacteria as promoters of plant growth for increased yield in landraces of African cereal crops. African J. Biotechnol., 3(1): 1-7.

Pandey, R.P; Nair, P.K.R; Tiwari, J.P. (1981). Correlation of morphophysiological and sink parameters in cow pea. Indian J. Agric. Sci. 51: 221-224.

Rangaswami, G; Oblisami, G. (1961). Quantitative studies on nitrogen fixation by five legume nodule bacteria. J. Annamalai Univ. 23: 119-124.

Singh, S.D. (1978). Effect of Rhizobiam inoculation on nodulation and yield of moong (Vigna radiata Wilczek). Ann. Arid Zone 16: 79-84.

Somasegaran, P. and Hoben, H.J. (1985). Methods in Legume-rhizobium Technology. NifTAL project and MIRCEN. Department of Agronomy, 2nd Soil Science Hawaii Institute Tropical Agriculture Human research, Univ Hawaii Manoa.

Subba-Rao, N.S., (1986). Phosphate Solubilization by soil Micro organisms. In: Advances in Agricultural Microbiology, Subba-Rao, N.S. (Ed.). Oxford and  IBH, New Delhi, pp: 295-303.

Vincent, J.M. (1970) A Manual for The Practical Study of Root-Nodule Bacteria. IBP Handb 15. Blackwell, Oxford, UK.

Wallace, D.M; Munger, H.M. (1965). Studies on the physiological basis for yield differences. I. Growth analysis of six dry bean varieties. Crop Sci. 5: 343-348.

Zahir, A.Z., Arshad, M. and Khalid, A., (1998).  Improving maize yield by inoculation with plant  growth promoting rhizobacteria. Pakistan J. Soil Science., 15: 7-11.