ISOLATION, IDENTIFICATION AND BIOASSAY OF NATIVE BACILLUS THURINGIENSIS ISOLATES AGAINST SPODOPTERA FRUGIPERDA

Suvathi Balu¹, Loveson Allwin²*, K. Elanchezhyan³, Vaithiyanathan Radhakrishnan⁴ Chandrasekaran Harisudan⁵, Manivannan MI ⁶ and Mariappan Paramasivan⁷

¹Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore,

Tamil Nadu, India

^2,3,6,7Agricultural College and Research Institute, Killikulam, TNAU, Tamil Nadu, India

⁴ Krishi Vigyan Kendra, Needamangalam,TNAU, Tamil Nadu, India

⁵Regional Research Station, Vridhachalam,TNAU, Tamil Nadu, India

Email: allwin.dr@gmail.com

Received-02.01.2024, Revised-16.01.2024, Accepted-27.01.2024

Abstract: Totally nineteen B. thuringiensis strains were isolated from 50 soil samples which were collected from various locations of Tirunelveli and Tuticorin districts. Out of 583 Bacillus like colonies 288 colonies were produced crystals and nineteen strains were identified as Btbased on crystal morphology. Bipyramidal, cuboidal, spherical shape of crystals and spores attached with crystals were observed in the isolates. All strains showed positive results to gram staining, motility test, Methyl Red (MR) test, catalase test. Some of the strains were negative for Voges Proskauer and Starch hydrolysis test.cry1, cry2Aa and cry2Abgenes were observed in these isolates through PCR analysis. KKM 2 and KKM 14 were caused above 60% mortality. KKM 5, KKM 17 and KKM 18 were showed lowest per cent mortality (16.67%) against S. frugiperda.

Keywords: B. thuringiensis, Isolation, Cry genes, Fall armyworm

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