Jolly Chauhan and Avadhesh Kumar Koshal*
Motherhood University, Roorkee, Haridwar, Uttarakhand PIN 247661
Email: akkoshal@hotmail.com
Received-04.01.2021, Revised-15.01.2021, Accepted-29.01.2021
Abstract: A methicillin resistant S. aureus (MRSA) screen tests solely for the presence of MRSA and no other microbes. This test is used to screen for MRSA in patient in hospitals. The first strain of penicillin resistant S. aureus was isolated in London, England hospitals. Staphylococcus is a versatile organism with several virulent characteristics andresistance mechanisms. Multidrug resistance is now the norm among the Gram-positive bacteria pneumococci, enterococci and staphylococci. S. aureus is perhaps the pathogen of greatest concern because of its intrinsic virulence, its ability to cause a diverse array of life-threatening infections in humans and in various animal species, and its capacity to adapt to different environmental conditions. In the present study, a total of 36 isolates of MRSA were obtained. All the suspected colonies were gram stained and plated on manitol salt agar. Growth of golden yellow colonies of Staphylococcus aureus on manitol salt agar plate. On gram staining gram-positive cocci in clusters were observed on all the slides. On manitol salt agar, small yellow manitol fermenting colonies were obtained. On MacConkey agar small light pink lactose fermenting colonies were observed. In the present study, the efficiency of the tube coagulase test can be markedly improved by sequel testing of the isolates with Mannitol salt agar, DNase and Tube coagulase. There is no single phenotypic test (including tube coagulase) that can guarantee reliable results in the identification of Staphylococcus aureus.
Keywords: Gram-positive, MRSA, Resistant strain, Staphylococcus, Virulent
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