P. Prasanna Lakshmi1*, A. Vijaya Gopal1, R. Lakshmipathy2 and P. Madhu Vani3
1Department of Agricultural Microbiology, Advance Post Graduate Centre, ANGRAU, Lam, Guntur
2Scientist, Agricultural Research Station, Amaravathi. 3Senior Scientist, Soil science,
RARS, Lam, Guntur
Received-11.05.2019, Revised-18.06.2019
Abstract: The isolation for nitrogen fixing Paenibacillus sppremains poorly explored. In this study, the endospore-forming Paenibacillus strains wereisolated from rhizospheric soil samples of sorghum collected from different places of Andha Pradesh. A total of twenty eight nitrogen fixing Paenibacillus strains wereisolated based on heat treatment at 70 0C for 10 minutes and growth on nitrogen free media. Two reference strains and all the twenty eight isolates took 3-4 days to show small to medium, circular, milky white colonies with entire margin. Morphology of two reference Paenibacillus strains and all the twenty eight isolates were found to be gram positive, endospore forming, rod shaped and without any pigmentation. The 28 Paenibacillus isolates and two reference strains were tested for different biochemical tests. Results revealed that 28 Paenibacillus isolates showed similar results to that of the reference stains. Therefore the 28 isolates were confirmed as Paenibacillus isolates.
Keywords: Rhizosphere, isolation, characterization, endospore, Paenibacillus spp.
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