Vishal kumar deshwal* and mohd tarik
Department of Microbiology, BFIT Group of Institution, Dehradun (India)
Email: vishal_deshwal@rediffmail.com
Received-07.09.2018, Revised-25.09.2018
Abstract: Aim of the present study was isolation and identification of Actinomycetes against certain pathogens. Actinomycetes strains were isolated from cultivated field of Sudhowala, Dehardun and Staphylococcus aureus, Escherichia coli, Salmonella typhi were also isolated from sewage at Dehradun. Both Actinomycetes and pathogens were characterized on the basis of microscopy and various biochemical tests. Further, we evaluated antimicrobial activity of Actinomycetes strains against isolated pathogens. Microscopic examination and biochemical tests confirmed that isolated strains were Actinomycetes, Staphylococcus aureus, Escherichia coli and Salmonella typhi. Actinomycetes did not show inhibition zone against Staphylococcus aureus. But crude extract of Actinomycetes showed 191.66, 181.81 % more inhibition zone as compare to 25% extract concentration against pathogenic E. coli and Salmonella typhi repectively. It confired that Actinimycetes effectively control growth of E. coli and Salmonella typhi.
Keywords: Actinomycetes, antibacterial, Staphylococcus aureus, Escherichia coli, Salmonella typhi
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