Akkenapally Snehanjali1, Shalini Mudalkar1*, Sreedhar Bodiga2, Reeja Sundaram1, Banda Sailatha1, Eega Kavya Sree1 and Rohith Ravula3
1Dept. of Forest Biology and Tree Improvement, Forest College and Research Institute,
Mulugu, Siddipet, Telangana
2Dept. of Basic and Social Sciences, Forest College and Research Institute,
Mulugu, Siddipet, Telangana
3Dept. of Silviculture and Agroforestry, Forest College and Research Institute, Mulugu,
Siddipet, Telangana
Email: shalini.mudalkar@gmail.com
Received-03.10.2022, Revised-11.10.2022, Accepted-23.10.2022
Abstract: The present investigation entitled “In vitro callus generation from nodal explant of Madhuca longifolia var. latifolia (Roxb.) A. Chev.” was conducted in the Tissue culture laboratory of Department of Forest Biology and Tree Improvement, Forest College and Research Institute, Mulugu, Siddipet during 2020-2022.The main objective of the present study was generation of callus from nodal explants. A protocol was developed for invitro propagation and indirect organaogenesis by using young nodal explants. The media was standardized and Callus generation was observed after four weeks on the surface of nodal explants. The nodal explants cultured on MS (Murashige and Skoog) supplemented with 0.5mg/L BAP + 0.1mg/L IAA had induced maximum calli response (26.00±0.40) and minimum calli response (10.50±0.64) in media of MS 0.25BAP + 0.1 IAA.
Keywords: In vitro callus, Madhuca longifolia, Investigation
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