Gibson Entuni1*, Rebicca Edward1 and Chyntia Jaby Entuni2
1Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300
Kota Samarahan, Sarawak, Malaysia
2Faculty of Engineering, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Email: gibsonentuni@gmail.com)
Received-08.01.2022, Revised-22.01.2022, Accepted-29.01.2022
Abstract: The over exploitation of natural resource such as tropical forest for deforestation and urbanization have threatened the population of endangered tropical timber tree species such as Eusideroxylon zwageri. The present study reported the results of a number of experiments aimed at optimizing shoot proliferation protocol for the mass propagation of E. zwageri. The effect of different combinations and concentrations of plant growth regulators (PGR) such as BAP, IBA and NAA on the shoot proliferation and elongation were evaluated. The findings showed that the best treatment for nodal segment multiplication in terms of the number of shoots and leaves per explant were obtained in the half strength MS medium supplemented with either 5.0 mg/L BAP alone or 5.0 mg/L BAP in combination with 0.5 mg/L IBA. Healthy shoots treated with 300 mg/L IBA for 20 min followed by transfer to another half MS medium without PGR resulted in 80.3% of shoots producing roots.
Keywords: Conservation, Timber, Borneo Ironwood, In vitro culture, BAP, Shoot elongation
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