M.K. Meena*, B.B.L. Thakore** and R.S. Jatav***
Department of Plant Pathology, Rajasthan College of Agriculture, Maharana Pratap University of Agriculture & Technology Udaipur- 3 13001
E mail : mkmeena879@gmail.com
* P.G. Scholar, Department of Plant Pathology, RCA, MPUA&T, Udaipur (Rajasthan)-313001
** Professor & Head, Department of Plant Pathology, RCA, MPUA&T, Udaipur (Rajasthan)-313001
*** Ph.D. Scholar, Department of Plant Pathology, RCA, MPUA&T, Udaipur (Rajasthan)-313001
Abstract : Studies were conducted in vitro to evaluation of different media, temperatures, lights and pH on growth and sporulation and production of toxic metabolites by Alternaria cyamopsidis causing leaf blight of cluster bean during 2003 at Department of Plant Pathology, Rajasthan College of Agriculture, Udaipur. Guar blight is more common disease in guar growing regions of Rajasthan and cause considerable losses (Singh, 1970). The optimum growth and sporulation of the pathogen were highly favorued in potato dextrose agar medium (88 mm) and potato dextrose broth medium (384.46 mg) and the temperature 30±2°C (78.67 mm) and pH 6.5 (830 mg) were more suitable for maximum growth and sporulation of the fungus. Good growth and sporulation were obtained in red and green light treatment. Crude toxin of the pathogen derived from 15 days old culture filtrate of A. cyamopsidis by which typical symptoms of blight were produced on guar leaves in bioassay test.
Keywords : A. cyamopsidis, Cluster bean, Cultural media, Light, pH, Temperature, Toxin
REFERENCES
Ayub, M. J. H. Mirza, and A. S. Shakir (1995). Physiological studies of Alternaria cyamopsidis Ranga. & Rao Causing leaf blight of guar [Cyamopsis tetragonoloba (L.)Taub.]. Pakistan Journal Phytopathology. 7.(1): 41-43.
Chandrashekar,M and M. C. Ball (1980). Leaf blight grey mangrove in Australia caused by .Trans. Brit. Mycol. Soc. 75(3): 413-418.
Dorn, M. (1954). Uterschunger Uberdes Auffreten Von sektoven in Pilzko Ionien (Insbesondereberi Alternaria tenuis) Arichiv fur Mikrobidogie. Bd. 21:310-328.
Khatun F., M. A. Hussain ,M. M. Hussain and D. K. Dey (1996). Effect of culture media ,pH,temperature and nitrogen sources on growth and sporulation of Bangladesh J.Pl. Pathol. 12 (1/2):29-32.
Gaur, H. N. and Dubey (1985). Effect of duration and phytotoxic metabolites from Verticillium dahliae on the cell membranes of cotton plants. Physiol. Plant Pathol. 27:108-118.
Kore, S.S. and R.L. Kharwade (1987). Fruit rot of round gourd caused by Fusarium oxysporum, Indian Phytopathology 40:251.
Lal B.B. and Chakravati (1979). Factors affecting the germination of sporangia of Physoderma maydis after infection of Maize leaves. Nowa Hedwigia 63: 91-96.
Li, H. R., B.C. Wu and S.Q. Yan (1998). Aetiology of Rhizoctonia in sheath blight of Maize in Sichuan, Plant Pathol. 47: 16-21.
Lilly, V. G. and H. L. Barnett (1951). Physiology of the fungi. Mc Graw Hill Book Co. Ltd., London.
Rangaswami G. and A.V. Rao (1957). Alternaria blight of cluster bean. Indian Phytopathology, 10: 18-25.
Singh, S.D. (1970). Studies on morphology, Physiology and control of Alternaria cyamopsidis causing leaf blight of gaur. M.Sc. Thesis University of Udaipur.