Amanpreet Singh Sran1, Jasvinder Kaur Sran2, Amandeep Kaur1, V.S. Pahil1
and Bahaderjeet Singh1*
1College of Agriculture, Guru Kashi University, Talwandi Sabo, Bathinda, Punjab.
2Department of Environmental Science, Nalanda Open University, Patna, Bihar
Email: sidhubahaderjit@yahoo.in
Received-01.06.2023, Revised-13.06.2023, Accepted-25.06.2023
Abstract: Potato is an important food crop worldwide. The black scurf disease caused by Rhizoctonia solani has emerged as a major problem in potato cultivation in recent years for farmers among the Punjab region. The present investigation was carried out in the Department of Plant Pathology Laboratory, Guru Kashi University, Talwandi Sabo during 2022. Studying the growth pattern of a pathogen in different culture media is a crucial aspect of understanding its behavior and characteristics. In this context, seven culture media viz., Potato dextrose agar, Sabouraud’s dextrose agar, Potato dextrose yeast agar, Oat meal agar, Czapek’s agar, Mushroom compost agar and Water agar medium were used to compare the growth rate of R. solani. Out of seven culture media tested, maximum mycelial growth was recorded in potato dextrose agar per plate while minimum mycelial growth was recorded in Water agar. Significantly maximum number of sclerotia was observed in Potato dextrose agar while minimum number of sclerotia was produced by Czapek’s agar medium. Moreover, no sclerotia was produced by R. solanion Water agar medium.
Key words: Potato, Culture media, Black scurf, Rhizoctonia solani
REFERENCES
Gupta, M. (2017). Studies on Rhizoctonia solani causing root rot of soybean, Thesis Submitted to the Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior.
Khan, I., Hussain, H., Shah, B., Ullah, W., Ahmad, N., Ali, W., Khan, N., Adnan, M., Junaid, K., Rizwan, S., Shah, A., Nazeer, A. and Iqbal, M. (2016). Evaluation of phytobiocides and different culture media for growth, isolation and control of Rhizoctonia solani in vitro. Journal of Entomology and Zoology Study, 4(2): 417-420.
Kumar, S., Kumar, A., Chand, G., Lal, M. and Kumar, R. (2014). Dynamics of mycelial growth and sclerotia production of Rhizoctonia solani Kuhn (AG1-IB) of urdbean. The Ecoscan, 8(3&4):273-277.
Lal, M. and Kandhari, J. (2009). Cultural and morphological variability in Rhizoctonia solani isolates causing sheath blight of rice. Journal of Mycology and Plant Pathology, 39(1):77-81.
Lalan, S., Sanjay, G. and Nagrale, D.T. (2013). Culture and physiological variability in Rhizoctonia solani, responsible for foliar and lesions on aerial part of soybean. Journal of Applied & Natural Sciences, 5:41-46.
Meena, P.D. and Chattopadhyay, C. (2002). Effect of some physical factors, fungicides on growth of Rhizoctonia solani Kuhn and fungicidal treatment on groundnut seed germination. Indian Journal of Plant Protection, 30(2):172-176.
Meera, G., Kumari, M. and Ruby, G. (2012). Effect of various media types on the rate of growth of Aspergillus niger. Indian Journal of Fundamental Applied Life Sciences, 2(2)141-144.
Sharma, L., Goswami, S. and Nagrale, D.T. (2013). Culture and physiological variability in Rhizoctonia solani, responsible for foliar and lesions on aerial part of soybean. Journal of Applied and Natural Science, 5(1):41-46.
Singh, P.K. and Singh, A.K. (2007). Studies on the suitability of different agar media on the growth of Rhizoctonia solani pathogen of rice. Flora and Fauna, 13(2):300-302.
Tribikram, S. (2016). Study of Root and Stem Rot Pathogen (Rhizoctonia solani) in Different Culture Media, Host Range and Effect of Weather Parameters on Disease Incidence. International Journal of Agriculture, Environment and Biotechnology, 13(3): 355-359.
Zhou, E., Yang, M., Li, L. and Zeng, W. (2002). The effects of media on the mycelial growth and sclerotial formation of Rhizoctonia solani AG-1-IA. Journal of South China Agriculture, 23(3):33-35.