Preeti Sirohi*
Department of Biotechnology, MNNIT Allahabad,Prayagraj-211004, Uttar Pradesh, India
Received-09.09.2019, Revised-27.09.2019
Abstract: The present study was undertaken to confirm the presence of genes responsible for resistance tobacterial leaf blight disease. Ninety-two rice genotypes evaluated for bacterial leaf blight resistance in the natural condition and under induced epiphytotic conditions. Four gene-specific primers were used to identify bacterial leaf blight resistant genes in these genotypes. Gene-specific markers linked to BLB resistant appeared as Xa4150bp (STS marker RM224) in 66 rice genotypes, xa51300bp (STS marker RG556) in 8 rice genotypes, xa131000bp (SNP marker RG136) in 73 rice genotypes and Xa211000bp (STS marker pTA248) in 32 rice genotypes. The seventy-one resistant genotypes had a varying number of BLB resistant genes. All four BLB resistance genes were detected in BC1F9 P16, BC2F7 P6,andBC2F7 P8 lines. Seventeen rice genotypes having a varying number of BLB resistance genes but unable to express resistance in the field conditions due to the effect of environmental factors. The lines BC1F9&BC2F7 involved in this study had xa13 in all the 38 lines (BC1F9 & BC2F7), while Xa21 was observed in thirty-one out of 38 lines. The lines BC1F9 & BC2F7 were found to be BLB resistant at molecular level & artificial induced condition. Application of back cross method was found successful to develop BLB resistant rice varieties using PR106 P2 as donor parent.
Keywords: Gene-specific markers, BLB, Epiphytotic conditions
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