2022, Issue 9, Volume 14

EXTRACTION AND PURIFICATION OF CARBOXYLESTERASES FROM THE SEEDS OF SAMANEA SAMAN

Krupa S.*,

 Chandan J.1, Gnanesh R.1, Lakshmikanth N.1, Rahul1 and Thejseh H.S.1

Department of Biochemistry, Jnanabharathi Campus, Bangalore University, Bangalore- 56

Jain University, J C Road, Bangalore.

Email: krupa.s@jainuniversity.ac.in

Received-03.09.2022, Revised-12.09.2022, Accepted-25.09.2022

Abstract: The enzyme carboxyl esterases was extracted from the Samanea samanseeds using different buffers at different pH. Highest activity was obtained with 33 mM phosphate buffer, pH 7.0. The enzyme assay was carried out using α-naphthyl acetate as substrate. The activity of carboxyl esterases was shown to have an optimal operating condition at pH 7.0 and a temperature of 50oC. The thermo stability of the enzyme was in the range of 7oC – 37oCwith the pH stability in the range of 4.0 – 8.0. The Km and Vmax values was determined as 0.157 mM and 1.785µM / minute. IC 50 of dicholorovas for Samanea saman esterase was found to be 2.23×10-10M. Dicholorovas was found to be an irreversible inhibitor as the time of incubation increase, the percentage of activity decreases.

Keywords: Carboxylesterases, Samanea saman, Dicholorovas, α – naphthyl acetate

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