N. Rajinimala1*, K. Elanchezhyan2, K. Kavitha3 and J. Sheela1
1 Department of Plant Pathology, Tamil Nadu Agricultural University, V.O. Chidambaranar Agricultural College and Research Institute, Killikulam
2 Department of Agricultural Entomology, Tamil Nadu Agricultural University, V.O. Chidambaranar Agricultural College and Research Institute, Killikulam
3 Krishi Vigyan Kendra, Tamil Nadu Agricultural University, Thirupathisaram
Email: rajinimala@tnau.ac.in
Received-12.06.2024, Revised-05.07.2024, Accepted-24.07.2024
Abstract: Meristem culture was followed to manage CMD caused by Cassava mosaic virus in cassava cv. MVD1. In meristem culture MS basal medium supplemented with 0.5 ppm of Benzyl aminopurine (BAP), 0.1 ppm of Gibberellic acid (GA3) and 0.1 ppm of Naphthalene acetic acid (NAA) was associated with the greatest callus induction of 95%, the highest shoot induction of 90% and the highest shoot elongation of 85% in cassava cv. MVD1. MS basal medium containing 0.5 ppm of BAP and 0.1 ppm of NAA was associated with maximum root formation in cassava cv. MVD1 (67.50 %). Absence of geminivirus in meristem derived cassava plants of cv. MVD1 was confirmed through Deng’s degenerate primer PCR.
Keywords: Cassava mosaic virus, Meristem culture, PCR, Deng primer
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