Rashmi R1*., M.V. Dhananjaya2, C. Aswath3 and Satish R. Patil4
1Horticulture, ICAR-KVK, Dakshina Kannada (KVAFSU, Bidar), Mangaluru, Karnataka, India
2Division of Vegetable Crops, ICAR-IIHR, Bengaluru, Karnataka, India
3Division of Floriculture and Medicinal Crops, ICAR-IIHR, Bengaluru, Karnataka, India
4Department of FLA, Kittur Rani Chennamma College of Horticulture, Arabhavi (UHS, Bagalkot) Karnataka, India
Email: rashmi.hortico@gmail.com
Received-08.03.2020, Revised-28.03.2020
Abstract: Gerbera is commercially multiplied through tissue culture using shoot tip explant and sub culturing frequently. This method allows for obtaining large amounts of healthy homogenous plants. However, frequent in vitro subculture leads to vitrification and reduced vigour in some populations. A study was therefore undertaken to investigate the effect of in vitro subcultures on vigour and confirmed that fifteen subcultures can be continuously performed from shoot tip without losing vigour with a span of 45 weeks. If this procedure is followed from 100 shoot tips, an approximately 47000 ready to plantlets can be obtained in a span of 45 weeks without losing vigour and quality. Quality planting material can be obtained at cheaper rate in the range of Rs.10-15 save the grower by 70% on planting material cost.
Keywords: Gerbera, Tissue culture, Shoot tip, Sub culture, Vigor
References
Amreen, T., Naik, H. B. and Sanathkumar, V. B. (2013). Performance of Gerbera genotypes under protected cultivation. Plant Archives., 13(2): 757-761.
Aswath, C. and Choudhary, M. L. (2001). Effect of cytokinin on the proliferation of multiple shoots in gerbera. Indian J. Hort., 58(4): 383-385.
Aswath, C. and Choudhary, M.L. (2002). Rapid plant regeneration f rom callus culture of Gerbera jamesonii. Acta Bot. Croat, 61(2): 125-134.
Daniells, J. (1997). Beware the potential hazards of tissue culture. Info Musa, 6(2): 17-18.
Jain, S. M. (2006). Mutation assisted breeding for improving ornamental plants. ISHS Acta Horticulturae, 714(1).
Kanwar, J. K. and Kumar, S. (2008). In vitro propagation of Gerbera- A Review. Hort. Sci. (Prague), 35(1): 35-44.
Murashige, T., Sepra, M. and Jones, J.B. (1974). Clonal multiplication of gerbera through tissue culture. Hort. Sci. 9: 175-180.
Naik, D., Vartak, V. and Bhargava, S. (2003). Provenance and subculture dependant variation during micropropagation of Gmelina arborea. Plant Cell Tissue Organ Cult., 73: 189-195.
Nair, A., Sujatha and Medhi, R.P. (2002). Performance of Gerbera cultivars in the Bay Islands. Indian Journal of Horticulture, 59(3): 322- 325.
Ray, T., Dutta, I., Saha, P., Das, S., Roy, S. C. (2006). Genetic stability of three economically important micropropagated banana (Musa spp.) cultivars of Indo- Ganjetic plains, as assessed by RAPD and ISSR markers. Plant Cell Tissue Organ Cult., 85: 11–21.
Vardja, R. and Vardja, T. (2001). The effect of cytokinin type and concentration and the number of subcultures on the multiplication rate of some decorative plants. Proc. Estonian Academy Sci. Biol. Ecology, 50(1): 22-32.