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Didhitee Patel¹, Ashwin Trivedi²* and Ghanshyam Patil³
1Dept. of Plant Physiology, Anand Agricultural University, Anand – 388110, Gujarat
2ICAR–Directorate of Medicinal & Aromatic Plants Research, Anand – 387310, Gujarat
3Centre of Excellence in Biotechnology), Anand Agricultural University (AAU),
Anand:388110, Gujarat
Received-26.09.2025, Revised-12.10.2025, Accepted-27.10.2025
Abstract: An efficient protocol was developed for plant regeneration, multiplication and rooting under in vitro condition in Coccinia grandis. Seed setting is low and seeds are pseudo due to parthenocarpic nature. So far, farmers in India have been cultivating tindora traditionally through the age-old asexual cuttings, which are often infected by diseases and pests such as thrips and aphids, and therefore give low yields. Micro propagated tindora offers clean disease-free planting material and thereby giving as much as 10 times higher yield than traditional propagules. The present experiment was conducted at Center for Advance Research in Plant Tissue Culture, Anand Agricultural University, Anand during 2018 – 2019. Combination of bavistin (2000 ppm) for 18 min., cefotaxime (1000 ppm) for 14 min., kanamycin (1000 ppm) for 14 min., streptocyclin (1000 ppm) for 18 min. and 0.1% HgCl2 for 8 min.for hard node explants, bavistin for 16 min., cefotaxime for 12 min., kanamycin for 12 min., streptocycline for 16 min. and 0.1% HgCl2 for 7 min. for soft nodal explants and bavistin for 10 min., cefotaxime for 6 min., kanamycin for 6 min., streptocyclin for 8 min. and 0.1% HgCl2 for 2 min. was found best for surface sterilization in the term of fungal contamination, bacterial contamination and drying of the all explants. Half strength of MS was the best initiation medium for all the explants. Treatment T6 took lesser number of days to sprouting and gave higher number of sprouted explants and response percentage at 7, 14 and 21 days after initiation. Half strength of MS supplemented with 0.1 BA mgl-1 was optimum for maximum multiplication of shoots. Half strength of MS supplemented with 0.1 mgl-1BA+1.0 mgl-1GA3 was optimum for maximum length of shoots. Half strength of MS supplemented with 1.0 IBA mgl-1 was optimum for maximum root length. Half strength of MS supplemented with 2.0 IBA mgl-1 was optimum for maximum number of roots. Coco peat was best suited for overall development of plants during primary hardening.
Keywords: Micro propagation, Parthenocarpic, Hardening
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