2020, Issue 4, Volume 12

VALIDATION OF MAS DERIVED LINES FOR INTROGRESSED GENE AGAINST BLAST AND BLB RESISTANCEIN SOUTHERN CHHATTISGARH

Prafull Kumar*

S.G. College of Agriculture and research Station, Jagdalpur, IGKV, Raipur, Chhattisgarh

Email: prafull397@gmail.com

Received-07.04.2020, Revised-29.04.2020

Abstract: The experiment was carried out at SGCARS, Jagdalpur, IGKV Raipur, Chhattisgarh to validate Marker Assisted Selection (MAS) derived genotypes, from ICAR-IIRR, Hyderabad, against blast and bacterial leaf blight resistance and access recurrent parent recovery. BPT 5204 (Samba Mahsuri) the recurrent parent 01 (RP 1) for the four test genotype recorded average plot yield of 4.00 kg/ha placing second in the experiment. When the recurrent parent 02 (Improved Sambha Mahsuri) was taken into account, genotype RP-Patho-1-2-15 recorded higher plot yield (4.23 kg). RP-Patho-1-2-15 and RP-Patho-3-56-11 were similar to the recurrent parent with heading spanof 78 and 79 DAS accordingly while RP-Patho-3-73-6 was six days in advance (70 days) to the recurrent parent (76 days). The entire test Near Isogenic Lines (NILs) with a plant height of 79-85 cm were similar to the recurrent parent (82 cm). Blast and bacterial leaf blight resistance gene carrying genotypes RP-Patho-2-18-5 and RP-Patho-2-16-4gave plot grain yield 3.77kg, which out yielded recurrent parent 02 but lesser than recurrent parent 01.Incidence of blast reported in Tetep (1-2%), C 101 LAC (8-10%)and average (5-8%) in all NILs.Blast resistance genes Pi 1 carrying genotypes RP-Patho-1-2-15 and RP-Patho-1-6-5, the infestation the comparatively higher (Score 6) than those with Pi 54 (RP-Patho-3-56-11 and RP-Patho-3-73-6, RP-Patho-3-56-11 and RP-Patho-3-73-6 (Score 4). However, dual genetic resistance background i.e. blast and bacterial blight resistance genes Xa 21+ Pi-54, provided excellent resistance even in hot spot centre for the disease. There was noincidence of Bacterial Leaf Blight (BLB)in all the isogenic line including donor and recurrent parents which may because of plant defence system or incompatible environmental condition for disease prevalence.

Keywords: NILs, Marker Assisted Selection, Recurrent parent, Blast, Bacterial Leaf Blight

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