Divya Vishambhar Kumbhakar1, Animesh Kumar Datta1*, Debadrito Das1, Bapi Ghosh1, Ankita Pramanik1 and Aditi Saha2
1Department of Botany, Cytogenetics, Genetics and Plant Breeding Section, University of Kalyani, Kalyani – 741235, West Bengal, India
2Department of Botany, NarasinhaDutt College, Howrah 711101
Email: dattaanimesh@gmail.com
Received-12.08.2016, Revised-26.08.2016
Abstract: Dry seeds(moisture content: 5.0%) of Nigella sativa L. (Family: Ranunculaceae; common name- black cumin, spice of commerce with immense therapeutic uses) are exposed to chemically synthesized copper (Cu) and cadmium sulphide (CdS) nanoparticles (NPs) at the doses of 0.25, 0.50 and 1.00 µg/ml for 3 and 6 h durations. EMS (ethyl methanesulphonate ̶ 0.25, 0.50 and 1.00%, 3 and 6 h durations) and gamma irradiations (25, 50, 100, 200 and 300 Gy; 60Co source) are used as positive control. The objective of the work is to foresee whether NPs can induce stable phenotypic mutation. The present communication highlights macromutation types and frequency, mutagenic efficiency and effectiveness and meiotic chromosome behaviour in treated materials and suggests the efficacy if NPs in inducing mutation in N.sativa and crop improvement.
Keywords: Cu- and CdS-NPs, Macromutants, Meiotic analysis, Mutagenic efficiency and effectiveness, Nigella sativa
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