D.S. Rajpoot1, Randhir Jha2, M.P. Goswami*3, Anu Jha4, Manisha Godbole5
and Anupama Goswami6
1 Shri Mukund biotech, Jabalpur
1,2,3IPS College, Chhindwara
3,6SFRI, Jabalpur India
Email: maneeshsfri@gmail.com
Received-09.05.2019, Revised-27.05.2019
Abstract: Fungi and bacteria contaminants were found associated with the cultured plant materials. The bacterial isolates include staphylococcus aureus, Gram positive, staphylococcus epidermis, staphylococcus aureus, Gram positive. And fungal contaminants include Fusarium sp., Aspergillus niger, chaetomium sp., Acremonium sp., Aspergillus sp. Microbes are living, biological contaminants that can be transmitted by infected people, animals and indoor air, and they can also travel through the air and get inside homes and buildings. It was discovered that the microbial population is higher in the preparatory room than the incubating rooms. This might be unconnected with the fact that more people frequent the preparatory room. Flaningan and Morey (1996), reported that presence of bacteria in a room indicate the presence of people and their levels may get high when the building is heavily populated. Consequently, adequately training of operators and high standards laboratory cleanliness is a vital pre-requisite to successful plant tissue culture. Most of these bacteria contaminants have been reported to increase culture mortality and the presence of latent infections can result in variable growth, tissue necrosis, reduced shoot proliferation and reduced rooting (Kane, 2003) The fungi were identified on the bases of morphological characteristic such as colony form and color, type of mycelium, fruiting bodies and spores (Commonwealth Mycological Institute Descriptions of Pathogenic Fungi and Bacteria. The present research study aims to a) examine the possible bacterial contamination of plant tissue cultures initiated from medicinal plants of Sinai, b) identify the bacterial species contaminating those plant cultures, c) determine the antibiotic sensitivity of those covert bacteria and d) suggest the best strategy to minimize loss of plant materials during short- and long-term cultures.
Keyword: Contamination, culture, Fungi, Bacteria
REFERENCES
Benjama, A. and Charkaoui, B. (1997). Control of bacillus contaminating date palm tissue in Micropopagation using antibiotics. In pathogen and microbial contamination using antibiotics. In Pathogen and microbial contamination management in Micropagation (Cassells A. C. Eds) Kluwer Dordrecht pp. 207 – 212.
Between Plant Tissue Cultures. In: Bacterial and Bacteria-like
Between Plant Tissue Cultures. In: Bacterial and Bacteria-like
Blake, J. (1994). Mites and Thrips As Bacterial And Fungal Vectors
Blake, J. (1994). Mites and Thrips As Bacterial And Fungal Vectors
Blake, J. (1994). Mites and Thrips As Bacterial And Fungal Vectors Between Plant Tissue Cultures. In: Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures ISHS Acta Horticulturae 225: http://www.actahort.org/books/225/index.htm.
Contaminants of Plant Tissue Cultures ISHS Acta Horticulturae 225:
Contaminants of Plant Tissue Cultures ISHS Acta Horticulturae 225:
Fellner, M., Kneifel, W., Gregorits, D. and Leonhardt, W. (1996). Identification and antibiotic sensitivity of microbial contaminants from callus cultures of garlic Allium sativum L. and Allium longicuspis Regel. Plant Science 113: 193-201.
Flannigan, B. and Morey, P.R. (1996). Control of moisture problems affecting biological indoor air quality. International Society of Indoor Air Quality and Climate, Ottawa, Canada, ISIAQ Guideline TF1-1996. http://www.isiaq.org/.
http://www.actahort.org/books/225/index.htm
http://www.actahort.org/books/225/index.htm
Kane, M. (2003). Bacterial and fungal indexing of tissue cultures. http://plant-c.cfans umn.edu/listerv/ 1996/log9612/indexing.htm
Kunneman, BPAM and Faaij-Groenen, GPM (1994). Elimination Of Bacterial Contaminants: A Matter Of Detection And Transplanting Procedures In: Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures Ishs Acta Hort. 225: 183 -188.
Stead, D.E., Hennessy, J. and Wilson, J. (1998). Modern methods for identifying bacteria. Plant Cell, Tissue and Organ Culture 52: 17-25.
Wildholm, J.M. (1996). The rare occurrence of plant tissue culture contamination by Methylobacterium mesophillicum. Plant Cell, Tissue and Organ Culture 45: 201-205.
Williamson, B., Cooke, DEL, Duncan, J.M., Leifert, C., Breese, W.A. and Shattock, R.C. (1998). Fungal infections of micropropagated plants at weaning: a problem exemplified by downy mildws in Rubus and Rosa. Plant Cell, Tissue and Organ Culture 52: 89-96.
Yang, S.H. and Kim, Y.M. (1989). Isolation and characterization of a pink-pigmented facultative methylotropic bacterium. Kor. Jour. Microbiol. March89: 63-69.