S.Vennila1*, K. Kumaran2, P. Radha2 and M. Mathivanan2
1Agricultural College and Research Institute, Vazhavachanur
2 Forest College and Research Institute, Mettupalayam
Tamil Nadu Agricultural University, Coimbatore
Received-02.08.2023, Revised-12.08.2023, Accepted-24.08.2023
Abstract: The present investigation was targeted to conduct the study of clonal propagation of Bixa orellana. This experiment consisted three types of cuttings viz., Leafy shoot cuttings, Soft wood cuttings and Semi hardwood cuttings and various concentrations of IBA viz., 1000 ppm, 2000 ppm, 3000 ppm, 4000 ppm, 5000 ppm, 6000 ppm and control. The experiment was replicated thrice with 50 cuttings in each treatment and was tested. The cuttings were planted in polythene bags (10 x 15 cm). The percentage of sprouting, rooting and survival differed significantly between treated and untreated cuttings. Semi hard wood cuttings treated with 4000 ppm of IBA maximized sprouting (92.05%) and rooting (78.34%) along with increased survival (68.13%). Survival and growth of the clonal plants were best when using improved techniques like types of cuttings with proper concentration of IBA and also combined with suitable rooting media. However, there was significant variation in shoot length, root length of cuttings due to IBA treatments. The results suggested that it could be possible to produce clones of high yielding superior genotypes of Bixa orellana on large scale through stem cutting for popularization and establishment of commercial forestry and agroforestry.
Keywords: Natural dye, Eco friendly, Bixa orellana,Clonal propagation, Mass multiplication
References
Ahmed, D.H. (1988). Preliminary report on macro propagation of Acacia albida. technique of propagation of forest trees. Tissue culture Vs mist propagation. Vanika sandesh,11:10-15.
Chauhan, J.M.S, Bisht, Prabha, Panwar, Monika and Thakur, Ajay (2015). In Vitro propagation of Elaeocarpus sphaericus. Indian Forester, 141(2): 173-177.
Hartmann and Kester’s (2011). Plant propagation: principles and practices. 8.ed. New York: Prentice-Hall, 915p.
Nanda, K.K. and Kochhar, V.K. (1984). Vegetative Propagation of Plants. Kalyani publishers, New Delhi. 234 p.
Parthiban, K.T., Surendran, Murugesh, M. and Buvaneswaran, C. (1999). Vegetative propagation of few multipurpose tree species using stem cutting. In: Advances in Horticulture and Forestry, 6 (199). Scientific Publishers, Jodhpur, pp 175-178.
Prabakaran, P., Umesh Kanna, S., Parthiban, K.T., Rajendran, P. and Mahendran, S. (2017). Macropropagation of Lannea coromandelica (Houtt.) Merr. Clones. Electronic Journal of Plant Breeding, 8(3), 764-771.
Reeves, K.K., Tomilson, H. and Leema, T. (1996). Vegetative propagation of Parkia biglandullosa. Journal of horticultural Science, 71 (2): 205-215.
Shah, V.N., Chauhan and Sood, R. (1994). Propagating Coriaria neplanensis, Woodfordia floribunda through stem cuttings. Van vigyan, 32: 102-107.
Singh, S. and Ansari, S.A. (2014). Mass multiplication of mature trees of Gmelina arborea Roxb. Through ex-vitro rooting of rejuvenated bud sprouts. Research Journal of Forestry. 8(1):25-31.
Sood, A., Paini, L.M.S., Sharma, M. and Sharma, O.P. (1994). Macropropagation of Dendrocalamus hamiltonii by using single node cuttings taken from elite seeding plants. Proc. 4 International Bamboo workshop in Asia and Pacific. Cniangima, Thailand.
Venketash, A. and Pandey, C.B. (2006). Rooting ability of padauk Pterocarpus dalbergioides Indian Journal of Forestry. 29 (2):131-133.
Vennila, S., Cinthia, C., Fernandaz and Kala, S. (2022). Standardization of clonal propagation of Pterocarpus indicus Willd. an important and endangered tree species. Journal of Biotechnology and Crop Science. 10 (15) January-December, 2022, 162-168.